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Refrigerator for liquified nitrogen biology

A liquid nitrogen and refrigerator technology, applied in applications, household appliances, household refrigeration devices, etc., can solve the problems of not meeting the requirements of the experiment and production process, high production technology requirements, and high operating costs, achieving outstanding environmental protection benefits, operation and maintenance. The effect of low cost and low maintenance cost

Inactive Publication Date: 2010-03-24
张磊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many similar patents, but they cannot meet the requirements of experiments and production processes
[0003] At present, due to the high technical requirements and high cost of production of this type of low-temperature refrigerator, most of them are foreign products, which consume a lot of power and make a lot of noise during use, so only some small experiments can be done, and the quantity is not large. The price is high, and the operating cost is also high. In order to overcome the shortcomings of using this type of low-temperature refrigerator in practice, the inventor has studied a liquid nitrogen biological refrigerator, which can meet the above biological test requirements, and the price is relatively cheap. Through the inventor efforts to complete the invention

Method used

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  • Refrigerator for liquified nitrogen biology
  • Refrigerator for liquified nitrogen biology
  • Refrigerator for liquified nitrogen biology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Place the cryopreservation tube 23 containing the fibroblast suspension that needs to be frozen in an ordinary refrigerator at 4° C. for 30 minutes, then transfer it to liquid nitrogen biological refrigerator storage room 2A and place it for 24 hours (set temperature -70°C), put it into the storage box 13, immerse it in the liquid nitrogen 8, and quickly match the temperature of the liquid nitrogen to -196°C, that is, the fibroblasts have been frozen.

Embodiment 2

[0031] Embodiment 2: Place the cryopreservation tube 23 containing the suspension of keratinocytes to be frozen in an ordinary refrigerator, and store it at 4°C for 30 minutes, then transfer the cryopreservation tube 23 to the storage room of a liquid nitrogen biological refrigerator, Set the temperature on the refrigerator control panel 26 to -30°C, freeze the tubes in the storage room of the refrigerator for 2 hours, then change the set temperature to -60°C, freeze for 4 hours, and then change the set temperature to - 90°C, freeze for 18 hours, take it out, put it into the storage box 13, close the storage box door 24, insert the storage box into the corresponding hole 19 of the heat insulation cover 6, immerse it in liquid nitrogen 8, and freeze it The tube 23 was quickly brought to a temperature of -196° C. with liquid nitrogen, and the refrigerator door 10 was closed to complete the freezing of the keratinocytes.

Embodiment 3

[0032] Embodiment 3: When the liquid nitrogen container liquid level indication alarm 12 alarms, the liquid nitrogen is transported from the supplier to the liquid nitrogen biological refrigerator with a Dewar bottle, and the liquid nitrogen biological refrigerator liquid nitrogen is added to the hole 15. The cork stopper 14 is opened, a funnel is placed, and the liquid nitrogen in the Dewar bottle is added to the stainless steel liquid nitrogen container 7 through the funnel until the liquid nitrogen 8 is added to a predetermined amount.

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Abstract

The invention relates to a refrigerator of liquid nitrogen biology, which is formed by thermal-insulated frame (1), stainless steel liquid nitrogen container (7), liquid level instructor alarm (12), thermal-insulated cover plate (6), storage bank (13), stainless steel ventilate tube (5), fan (4), storage room, temperature sensor, and temperature controller (26); the refrigerator at -70--196Deg. Ccan store cell, organism block, and nucleic acid, with simple structure, lower cost, low power consumption and simple maintenance.

Description

technical field [0001] The invention relates to a liquid nitrogen refrigerator, in particular to biological and medical laboratories used for storage of cells, tissue blocks, nucleic acids and other necessary equipment at low temperatures at about -70°C or lower. Background technique [0002] Low-temperature refrigerators (below -70°C) or liquid nitrogen biological containers are necessary equipment for storing cells, tissue blocks, nucleic acids, etc. in laboratories. At present, there are few domestic refrigerators with refrigerators below -25°C. Refrigerators for biological experiments are even rarer. Generally, only liquid nitrogen is used as a refrigerant to reach this temperature. The heating devices used in biological refrigeration centrifuges or refrigerators used in industry are more complicated and expensive. In terms of patents, such as application The number is CN200420017227.9 and the title "Small Low-Temperature Biological Freezer" is introduced as a small-scal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): F25D3/10
Inventor 张磊李娟
Owner 张磊
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