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Photosynthetic bacterium enriched culture medium

A technology of photosynthetic bacteria and culture medium, which is applied in the field of culture medium for enriching photosynthetic bacteria, can solve the problems of long cycle, high cost, and many types of medium components, and achieve the effects of low cost, fast growth and fast cultivation

Inactive Publication Date: 2009-12-02
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The key to the promotion and application of photosynthetic bacteria is the need for a suitable culture medium, but the current classic conventional medium for enriching photosynthetic bacteria, the main formula is: ammonium chloride 1g, sodium bicarbonate 1g, dipotassium hydrogen phosphate 0.2g , sodium acetate 1-5g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.5-2g, growth factor 10ml, trace element solution 10ml, distilled water 980ml, (see "Microbiology Experiment Tutorial"), the average enrichment time is 2 to 8 weeks, the cycle is long, and there are many types of medium components, and the disadvantages of high cost

Method used

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Experimental program
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Effect test

Embodiment 1

[0022] (1) Preparation of Enriched Photosynthetic Bacteria Culture Medium

[0023] Basal medium: 1.0g ammonium chloride, 1.0g sodium bicarbonate, 0.2g dipotassium hydrogen phosphate, 3.0g sodium acetate, 0.2g magnesium sulfate heptahydrate, 1.0g sodium chloride, made into 1000ml with distilled water.

[0024] In the above basal medium, add 0.1 g of yeast extract and 0.5 g of hydroxysuccinic acid.

[0025] (2) The cultivation of photosynthetic bacteria

[0026] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Photosynthetic bacteria (commercially purchased) were inoculated in the culture medium at an inoculation concentration of 4%, oscillated and mixed, and placed in a light incubator for light culture under the light conditions of 1500 lux and temperature of 30°C.

[0027] Enrichment culture results: After 5 days of light culture, the bacterial solution OD 660 1.3, after 8 days of light culture, the maximum growth amount was reached, and the O...

Embodiment 2

[0029] (1) Preparation of Enriched Photosynthetic Bacteria Culture Medium

[0030] Basal medium: 1.0g ammonium chloride, 1.0g sodium bicarbonate, 0.2g dipotassium hydrogen phosphate, 3.0g sodium acetate, 0.2g magnesium sulfate heptahydrate, 1.0g sodium chloride, made into 1000ml with distilled water.

[0031] In the above basal medium, add 3.0 g of yeast extract and 2.0 g of hydroxysuccinic acid.

[0032] (2) The cultivation of photosynthetic bacteria

[0033] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Photosynthetic bacteria (commercially purchased) were inoculated in the culture medium at an inoculation concentration of 4%, oscillated and mixed, and placed in a light incubator for light culture under the light conditions of 1500 lux and temperature of 30°C.

[0034] Enrichment culture results: After 5 days of light culture, the bacterial solution OD 660 1.4, after 10 days of light culture, the maximum growth amount was reached, and the ...

Embodiment 3

[0036] (1) Preparation of Enriched Photosynthetic Bacteria Culture Medium

[0037] Basal medium: 1.0g ammonium chloride, 1.0g sodium bicarbonate, 0.2g dipotassium hydrogen phosphate, 3.0g sodium acetate, 0.2g magnesium sulfate heptahydrate, 1.0g sodium chloride, made into 1000ml with distilled water.

[0038] In the above basal medium, add 1.0 g of yeast extract and 1.5 g of hydroxysuccinic acid.

[0039] (2) The cultivation of photosynthetic bacteria

[0040] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Photosynthetic bacteria (commercially purchased) were inoculated in the culture medium at an inoculation concentration of 4%, oscillated and mixed, and placed in a light incubator for light culture under the light conditions of 1500 lux and temperature of 30°C.

[0041] Enrichment culture results: After 5 days of light culture, the bacterial solution OD 660 1.8, after 12 days of light culture, the maximum growth amount was reached, and the ...

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Abstract

The invention relates to an enrichment culture medium of photosynthetic bacteria. The technic proposal is that: the enrichment culture medium of the photosynthetic bacteria is characterized in that basic culture medium, yeast extract and malic acid are contained, wherein, 0.1g-3.0g of the yeast extract and 0.5g-2.0g of the malic acid are added to each 1000ml of the basic culture medium. The photosynthetic bacteria cultivated by the culture medium of the invention has rapid cultivating speed and low cost, makes up the defects of over short growth period of the photosynthetic bacteria and over quick increase of pH, etc. during the propagation process, therefore, the growth period is prolonged by 5-7 days and the maximum growth quantity OD 660 is increased to 1.6-3.0.

Description

Technical field: [0001] The invention relates to a culture medium for enriching bacteria, in particular to a culture medium for enriching photosynthetic bacteria. Background technique: [0002] Photosynthetic bacteria is a general term for a large group of prokaryotic organisms capable of photosynthesis. The practice of research and application shows that photosynthetic bacteria can use a variety of sulfides and organic matter as hydrogen donors and organic carbon sources for photosynthesis, and can be used in the treatment and recycling of high-concentration organic wastewater, water quality regulation and promotion of healthy growth in aquaculture, As a high-efficiency active bacterial fertilizer in agriculture, it plays a very beneficial and remarkable role. [0003] Photosynthetic bacteria is a general term for a special physiological group of prokaryotic microorganisms that do not produce oxygen. They are the earliest prokaryotic microorganisms with a primitive photosy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38
Inventor 马溪平潘玲孙学凯付宝荣徐成斌李法云冷阳
Owner LIAONING UNIVERSITY
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