Drynaria fortunei breeding method
A Quercus fern and spore technology is applied in a breeding field of Quercus fern.
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Embodiment 1
[0021] The spore culture of embodiment 1, oak fern
[0022] Medium:
[0023] 1 / 2 MS medium: the macronutrients and trace elements are 1 / 2 of the full amount of MS medium, the concentration of iron salt and organic components is the same as MS medium, 0.5% (W / V) agar, pH 5.8. After boiling, it was divided into 100mL Erlenmeyer flasks, and then sterilized by high-pressure steam at 121°C for 20min.
[0024] 1. Sterilization of Quercus fern spores
[0025] Take 5mg of mature Quercus fern spores and put them in a 1.5mL centrifuge tube, drop them into sterile water, oscillate fully to make a suspension, let stand for 2 hours, centrifuge at 4000r / min for 1min, discard the supernatant; then drop about Disinfect and sterilize the spores with 1.2 mL of 5% NaClO solution (each 100 mL of this solution contains 0.1 mL of Tween-80). After 4 min, centrifuge at 4000 r / min for 1 min, discard the supernatant; finally rinse with sterile water for 4-5 Once, a sterile spore suspension was obtai...
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