Industrial production of Huan-Cao dendrobe (dendrobium loddigessi Rolfe) technique and culturing method therefor
A technology of Dendrobium eccentricum and production technology, which is applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems that restrict the industrialization and large-scale development of authentic medicinal material Dendrobium eccentricum, and shorten the time of tissue culture. Time and cost, good social and economic benefits, authenticity effect
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Embodiment 1
[0026] Embodiment 1: rapid propagation technology of Herba Dendrobii seedlings
[0027] After the mature capsules of the wild Dendrobium candidum are harvested, they are placed in a clean petri dish, and after they crack naturally, the seeds are put into a clean beaker and sealed, and stored in a refrigerator.
[0028] Put fresh uncracked fruit or stored seeds through a certain disinfection procedure, sow 1 / 2 MS basic medium + NAA (a-naphthalene acetic acid) 0.5 mg / L + banana taro juice 20% (V / V) + In the medium of agar 5g / L, carry out the seed germination culture, the culture temperature is 24 ℃, the light is 9 hours a day, the light intensity is 2500lx, the seed embryo starts to germinate in about 12 days, the seed embryo expands in 20-30 days, and gradually germinates to form a green protocorm;
[0029] Transfer the protocorm to 1 / 2 MS basic medium + BA (6-benzyl adenine) 0.5 mg / L + NAA (a-naphthalene acetic acid) 0.2 mg / L + canna juice 20% (V / V) + agar 5g / L medium for p...
Embodiment 2
[0031] Embodiment 2: the seedling hardening technique of tissue culture seedling of Herba Dendrobii
[0032] Seedling hardening substrate is small bluestone (3cm-5cm) + humus soil + moss, ratio: 5:3:2 (thickness);
[0033] Seedling hardening method: use a 1.5m×22m seedbed, spread 5cm of small bluestone, 3cm of humus soil, and 2cm of moss on the seedbed. After all are sterilized, take out the seedlings in the culture bottle and wash them, and then sterilize them in Duojingling After 5-10 minutes, the seedlings are graded and hardened again, and 3-5 plants are taken as a clump, and the roots are planted in the humus soil under the moss, and then it is advisable to pour root-fixing water once.
[0034] Conditions for hardening seedlings: Generally, warm sheds are used for hardening seedlings, and the temperature in the sheds is relatively controlled. In order to adapt to the conditions for transplanting in the future, constant temperature conditions should not be used for the tem...
Embodiment 3
[0035] Embodiment 3: Dendrobium candidum transplanting technique
[0036] Transplanting medium: bluestone ranging from 3cm to 8cm + humus soil 3cm + moss, the thickness ratio is: 8cm: 3cm: 2cm;
[0037] Transplanting conditions: ① Outside the shed, rocky mountainous areas with better shading, generally below the mountainside, in addition to retaining wild moss conditions, artificial planting seedbeds can be used. Spread a 1cm wide cultivation bed in the open space, with 40cm intervals. The base can be selected at an altitude below 1200m. Shade cloth is used for shading, the temperature is mainly in nature, the humidity is between 60%-85%, the shading degree reaches 60%, and the light is about 2000-3000lx.
[0038] ② Cultivation in sheds, using plastic greenhouses, using seedbeds or cultivation, the seedbed size is 1.5m×22m. The scattered light in the shed reaches more than 40%, about 1500-2800lx. The temperature can be between 5°C and 35°C, and the humidity should not be l...
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