Sensor for detecting phenolic substance in compost and detection method thereof
A phenolic substance and sensor technology, which is applied in the field of biosensor determination of phenolic substances in complex composting systems
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[0042] 1. Laccase-Fe 3 o 4 Preparation of magnetic nanoparticle cross-linked body
[0043] Under the protection of nitrogen, a certain amount of Fe 2+ : Fe 3+ = 1:2 ratio of FeCl 2 , FeCl 3 Add 38mL 0.4M hydrochloric acid to dissolve, then pour into 375mL 0.7M ammonia water, stir vigorously to form Fe 3 o 4 Colloidal precipitate. Then take 0.232g of the precipitate, add it to 200mL of n-propanol, and then add 5.36g of PEG (polyethylene glycol) and 20mL of H 2 O, 10mL ammonia water and 1.2mLTEOS (tetraethyl orthosilicate), stirred for 24h, separated, washed, and freeze-dried, suspended in 10mL methanol with 0.5g, added 0.5mL APTMS (aminopropyltrimethoxysilane), stirred at room temperature Silanization was completed within 12 hours, and then suspended in 5 mL of 2.5% glutaraldehyde and stirred to obtain aldylated core / shell magnetic nanoparticles. Then at 4 °C, 0.01 g laccase and 0.5 g magnetic nanoparticles were added to 5 mL of 1 / 15M Na 2 HPO 4 and 1 / 15MKH 2 PO 4 ...
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