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Method of making longan seed extract

a technology of longan seed and extract, which is applied in the field of extraction techniques, can solve the problems of compromise the survival of the organism, side effects, and death in some cases, and achieve the effects of healing wounds, reducing side effects, and reducing side effects

Inactive Publication Date: 2015-03-19
JOBEN BIO MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention relates to a method for extracting longan seeds which have various beneficial effects such as curing inflammation, treating hyperuricemia, healing wounds, and preventing microorganism growth.

Problems solved by technology

Similarly, progressive destruction of the tissue would compromise the survival of the organism.
However, side effects are also reported.
It is fatal in some cases.

Method used

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  • Method of making longan seed extract
  • Method of making longan seed extract
  • Method of making longan seed extract

Examples

Experimental program
Comparison scheme
Effect test

experiment 1

[0068]Treatments with longan seed extract obtained from a solvent having 50% of ethanol, longan seed extract A obtained from a solvent having 100% pure water, and longan seed extract obtained from a solvent having 20% of ethanol improve inflammation symptoms as discussed below.

[0069]24 Sprague Dawley (SD) rats are grouped into nine groups of different SD rats in which one group is chosen as control group and the remaining groups chosen as treatment groups. It is noted that all SD rats are male in the invention. Each SD rat has a weight of 200-250 g. Room temperature is kept at 23° C. Room is kept bright for 12-hour and dark for next 12-hour repeatedly. Water is treated by reverse osmosis.

[0070]SD rats in the control group is fed with water only.

[0071]Longan seed extract is oral fed to the SD rats of a first treatment group in the weight of 0.5 g / Kg of SD rat. After one week, LPS (lipopolysaccharides) of 2.5 mg / Kg of SD rat is abdomen injected into each SD rat. Another one day is wai...

experiment 2

[0081]Treatments with longan seed extract obtained from a solvent having 50% of ethanol improve gout symptoms as discussed below.

[0082]24 SD rats are grouped into three groups of eight SD rats in which first group is chosen as control group, second group chosen as treatment group, and third group chosen as longan seed group. Each SD rat has a weight of 200-250 g. Room temperature is kept at 23° C. Room is kept bright for 12-hour and dark for next 12-hour repeatedly. Water is treated by reverse osmosis.

[0083]SD rats in the control group is fed with water only.

[0084]Hypoxathine in the weight of 300 mg / Kg of SD rat and oxonic acid in the weight of 250 mg / Kg of SD rat are oral fed to the SD rats of the treatment group. Hypoxathine in the weight of 300 mg / Kg of SD rat, oxonic acid in the weight of 250 mg / Kg of SD rat, and longan seed of 0.1 wt % are oral fed to the SD rats of the longan seed group.

[0085]After one night of abstaining from food and drink, ether as anesthesia agent is admin...

experiment 3

[0087]50 mmol / L of xanthine is prepared by using a buffer solution called PBS (phosphate buffered saline). 0.1-0.2 unit / ml of xanthine oxidase is prepared by using PBS. Following samples are prepared: (1) Preparation of pure water and preparation of longan seed extract therefrom. (2) Preparation of solution having 20% ethanol and preparation of longan seed extract therefrom. (3) Preparation of solution having 50% ethanol and preparation of longan seed extract therefrom. (4) Preparation of solution having 95% ethanol and preparation of longan seed extract therefrom. Allopurinol is taken as a positive control group. Xanthine oxidase is added to the control group. After five minutes, xanthine is added to the control group. Water is added to a blank control group. Xanthinie oxidase is added to each sample. After five minutes, xanthine is added to each sample. A spectrometer is used to emit light of wavelength of 290 nm to impinge on the samples and the control groups. Light absorption c...

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Abstract

A method of making longan seed extract is provided with choosing an extraction solvent; heating the extraction solvent to a first predetermined temperature; adding pulverized longan seed to the extraction solvent to prepare a solution; maintaining the solution at a second temperature for a predetermined period of time to obtain an extracted substance; filtering the extracted substance; and drying and cooling the filtered extracted substance to produce an extract.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This nonprovisional utility application is a divisional application of and claims the benefit under 35 U.S.C.§120 to co-pending U.S. application Ser. No. 13 / 382,907 filed Mar. 8, 2012, all of which are incorporated, in their entirety, by this reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The invention relates to extraction techniques and more particularly to a method of making longan seed extract.[0004]2. Description of the Related Art[0005](1) Inflammation is part of the complex biological response of vascular tissues to harmful stimuli, such as pathogens, damaged cells, or irritants. Inflammation is a protective attempt by the organism to remove the injurious stimuli and to initiate the healing process. Inflammation is not a synonym for infection, even in cases where inflammation is caused by infection. Although infection is caused by a microorganism, inflammation is one of the responses of the organism to th...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/77
CPCA61K36/77A61P17/02A61P19/06A61P29/00A61P31/00A61P31/04Y02A50/30
Inventor TSENG, HUANG-CHUNGHOU, CHIEN-WEI
Owner JOBEN BIO MEDICAL
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