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System and Method for Ultrasonic Sample Preparation

a technology of ultrasonic sample and preparation method, which is applied in the field of system and method of ultrasonic sample preparation, can solve the problems of significant drawbacks of the process, varies the amount of ultrasonic energy received in each sample well, and the energy transmitted by the ultrasonic probe into the liquid bath is not uniformly distributed throughout the bath

Inactive Publication Date: 2015-02-05
SONICS & MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is about a new system and method for ultrasonic sample preparation that avoids problems like unpredictable energy transmission and varying energy dispersion. It also ensures that mechanical vibrations transmitted by the ultrasonic probe are evenly distributed to each sample in the microplate. This results in more efficient and uniform sample preparation.

Problems solved by technology

This process has significant drawbacks, however.
First, the energy transmitted into the liquid bath by the ultrasonic probe is not transmitted uniformly throughout the bath.
As a result of this non-uniform dispersion of energy, the amount of ultrasonic energy received in each sample well varies.
Another disadvantage with the above described system and method for sample preparation is that the magnitude and duration of ultrasonic energy transmitted to different parts of the bath is unpredictable.
Therefore, even if the non-uniform dispersion characteristics of the bath could be accounted for in a preparation protocol, the unpredictable nature of the energy transmission introduces unaccountable error.
Another disadvantage with the above described system and method is that the energy emitted by the probe is highly focused within the bath.
As a result, the results of such assays are unreliable.

Method used

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  • System and Method for Ultrasonic Sample Preparation
  • System and Method for Ultrasonic Sample Preparation
  • System and Method for Ultrasonic Sample Preparation

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Embodiment Construction

[0037]In reference to FIGS. 1-2, a system 10 for ultrasonic sample preparation is shown. The system 10 includes vessel 20. It should be noted that a front face of the vessel 20 is not shown in FIG. 1 in order to more fully illustrate the present invention through FIG. 1. The vessel 20 has a wall 21 that defines an inner volume 22. The inner volume 22 is configured such that a liquid 24 can be received and contained therein. In FIG. 1, liquid water 24 is received in the inner volume 22. It should be understood, however, that one or more different liquids may be received in the inner volume 22 of the vessel 20. The liquid 24 facilitates transmission of mechanical vibrations emitted from an ultrasonic probe 40 (discussed in detail below) to a sample 34 (discussed in detail further below).

[0038]The system 10 includes a microplate 30 disposed in the inner volume 22 of the vessel 20. The microplate 30, also known as a Microtitre plate or microwell plate, is a flat plate with multiple “sam...

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Abstract

A system and method for ultrasonic sample preparation, includes a vessel having a wall defining an inner volume. An ultrasonic probe is disposed in the inner volume of the vessel. A microplate having a plurality of sample wells is also disposed in the inner volume of the vessel. An actuator is connected to the microplate and is configured to move the microplate relative to the ultrasonic probe in the inner volume to facilitate uniform distribution of ultrasonic energy.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the use of ultrasonic energy to process materials. In particular, the present invention relates to a system and method for preparing sample materials using ultrasonic energy.BACKGROUND OF THE INVENTION[0002]It is well known to use ultrasonic energy to prepare sample materials for diagnostic investigations. For example, Chromatin Immunoprecipitation (ChIP) assays, a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell, uses ultrasonic energy.[0003]The ChIP technique involves: (1) crosslinking proteins with DNA; (2) fragmenting (also referred to as shearing) the chromatin (cross linked DNA-protein complex) from the cell; (3) preparing the soluble chromatin; (4) immunoprecipitating the protein of interest; and (5) identifying the segment associated with the protein. The fragmenting step is typically performed by transmitting ultrasonic energy to cells via ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806B06B3/00G01N1/38B01F31/201B01F31/87
Inventor DONATY, MICHAEL
Owner SONICS & MATERIALS
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