Novel regulatory elements of cold-inducible hutU gene from the Antarctic psychrotrophic bacterium Pseudomonas Syringae

Abstract

A DNA sequence from the upstream region of cold-inducible hutU gene of the Antarctic Psychrotrophic Bacterium Pseudomonas Syringae, comprising promoter elements and other regulatory sequences, with unique 'CAAAA' nucleotide sequence at -10 site of multiple transcription start sites and using said promoter to express genes of interest in the said bacterium at temperature as low as 4° C. and using the said bacterium with generation time ranging between two and half to three hours, as a system to produce low temperature labile proteins of pharmaceutical significance.

Description

[0001] A DNA sequence from the upstream region of cold-inducible hutU gene of the Antarctic Psychrotrophic Bacterium Pseudomonas Syringae, comprising promoter elements and other regulatory sequences, with unique `CAAAA` nucleotide sequence at -10 site of multiple transcription start sites and using said promoter to express genes of interest in the said bacterium at temperature as low as 4.degree. C. and using the said bacterium with generation time ranging between two and half to three hours, as a system to produce low temperature labile proteins of pharmaceutical significance.BACKGROUND AND PRIOR ART REFERENCES[0002] Antarctic bacteria provide a useful model system for studying cold adaptation (15, 17, 31, 36). These organisms are generally represented by the psychrotrophs and psychrophiles, which have the ability to grow at 0.degree. C. They can transcribe at this lower temperature both in vitro and in vivo (31). However, nothing much is known about the nature of promoter and regu...

AI Technical Summary

Benefits of technology

0011] A DNA sequence from nucleotide 2961 to 3600 of the upstream region of cold-inducible hutU region of the Antarctic Psychrotrophic Bacterium Pseudomonas Syringae of accession No.AF326719, comprising promoter elements and other regulatory sequences, with unique `CAAAA` nucleotide sequence at -10 site of multiple t

Problems solved by technology

However, nothing much is known about the nature of promoter and regulatory elements from these bacteria or about the mechanism of transcription at lower temperatures.

However, absolutely no information is available with regard to the characteristics of promoter sequence such as the -10 and -35 elements from the bacterium for such low temperature specific transcription.

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