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Selective breeding method for rape cytoplasmic male sterile line

A technology of male sterile lines and male sterility, applied in the fields of botany equipment and methods, application, plant gene improvement, etc., to achieve the effect of improving breeding effect, clear breeding purpose and simple breeding procedure

Inactive Publication Date: 2011-03-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main disadvantage of Pol cms is that the fertility of the sterile line is affected by the degree of temperature due to the difference in the nuclear gene background, that is, fertile pollen appears under low or high temperature conditions, which affects the breeding effect

Method used

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  • Selective breeding method for rape cytoplasmic male sterile line
  • Selective breeding method for rape cytoplasmic male sterile line
  • Selective breeding method for rape cytoplasmic male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The cultivation of embodiment 1 mustard type rape cytoplasmic male sterile line

[0032] 1. The steps of genetic classification and molecular marker-assisted breeding of cytoplasmic male sterile lines of Brassica napus are as follows:

[0033] 1. Determination of recovery relationship:

[0034] (1) The mustard-type cytoplasmic male sterile lines were genetically classified through the determination of the restoration relationship, and 6-102A was identified as a new sterile cytoplasmic type.

[0035] (2) Through the determination of the recovery relationship (see literature: Fu Tingdong, "Breeding and Utilization of Hybrid Rapeseed", Hubei Science and Technology Press, 2000), select 39 varieties or lines of Brassica napus and Brassica napus respectively with pol CMS; ogu CMS, tour CMS, nap CMS, and 6-102A rapeseed cytoplasmic male sterile materials were used for test crossing, and the results of the restoration relationship determination are shown in Table 2:

[0036] ...

Embodiment 2

[0058] Example 2 Obtaining the Cytoplasmic Male Sterile Line of Brassica napus by Backcross Breeding

[0059] According to the breeding steps described in "two" in Example 1, the cytoplasmic male sterile line 6-102A of Brassica napus was used as the female parent, and the Brassica napus variety Huashuang No. 3 (a conventional double-low rape variety publicly used) was used as the female parent. As the recurrent parent, the cytoplasmic male sterile line of Brassica napus was obtained through eight consecutive generations of backcross selection. The propagation procedure of the cytoplasmic male sterile line is the same as that of the mustard rape cytoplasmic male sterile line 6-102A.

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Abstract

The invention relates to a method for selecting male sterility line of rape cytoplasm, wherein the invention comprises that: selecting wide mustard sterility male, using mustard rape as hold line; crossing and molecule mark to assist the selection, to obtain the mustard rape cytoplasm male sterility line and hold line with stable generation; cultivating said sterility line to obtain usable mustard rape cytoplasm male sterility line.

Description

Technical field [0001] The invention is the field of rape breeding technology, which involves a new breeding, reproduction and utilization of a new rapeseed cytoplasm male infertility system. Background technique [0002] Cyclical male infertility is the most important way for rapeseed hybrid advantages.At present, there are many types of cytoplasm male infertility that have been reported at home and abroad. According to different sources, it can be divided into two categories: heterologous cytoplasm male infertility and homologous cytoplasm male infertility: in heterogeneous CMS, the most useful value has begunCommercial breeding is OGU CMS, Kosena CMS, and Tour CMS; in the homologous CMS, the Boloma cytoplasm male infertility (POL CMS) with commercial utilization value.POL CMS is Academician Fu Tingdong of Huazhong Agricultural University discovered and invented the first internationally internationally the first internationally valuable rapeseed Boli Maxiong.It is still the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00A01H1/02A01H1/04
Inventor 傅廷栋涂金星马朝芝万正杰李兴华
Owner HUAZHONG AGRI UNIV
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