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Method for breeding male sterile series of envelopped senvy cytoplasm

A technology of male sterile line and mustard greens, applied in the directions of botanical equipment and methods, introduction of foreign genetic material using vectors, plant genetic improvement, etc., to achieve the effect of stable sterility

Inactive Publication Date: 2009-07-29
HUAZHONG AGRI UNIV
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of the existing cytoplasmic male sterile line of mustard mustard, propose a new method of stable fertility of mustard cytoplasmic male sterile line, and provide a new method for the utilization of heterosis of mustard vegetable. new germplasm resources

Method used

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  • Method for breeding male sterile series of envelopped senvy cytoplasm
  • Method for breeding male sterile series of envelopped senvy cytoplasm
  • Method for breeding male sterile series of envelopped senvy cytoplasm

Examples

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Embodiment 1

[0030] Example 1 Breeding of Cytoplasmic Male Sterile Line of Brassica juncea

[0031] One, the cytoplasmic male sterile line 6-102A of mustard type rape is carried out genetic classification, and its steps are as follows:

[0032] 1. Determination of recovery relationship:

[0033] (1) The correct genetic classification of the mustard-type cytoplasmic male sterile line was carried out through the determination of the restoration relationship, and the material numbered 6-102A was determined to be a new cytoplasmic male sterile cytoplasmic type.

[0034] (2) Through the determination of the recovery relationship (see Fu Tingdong, "Breeding and Utilization of Hybrid Rapeseed", Hubei Science and Technology Press, the method introduced in 2000 edition), select 39 Brassica napus and Brassica napus varieties or lines respectively Testcross with pol CMS; ogu CMS, tour CMS, nap CMS, 6-102A, and the results of the restoration relationship are shown in Table 1.

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Abstract

The invention belongs to the technical field of cruciferous vegetable breeding, in particular, the invention belongs to a method for breeding a cytoplasmic male sterile line of mustard mustard. The method is characterized in that the natural mustard-type rapeseed cytoplasmic male sterile line 6-102A is selected as the female parent, the maintainer mustard 6-150 is used as the male parent, and genetically stable mustard mustard is obtained through continuous backcrossing and molecular marker-assisted selection Cytoplasmic male sterile line and maintainer line, the sterile line is propagated to obtain the cytoplasmic male sterile line CMS6-150 of mustard mustard which can be used for production. The cytoplasmic male sterile line of mustard mustard has the same cytoplasmic type as 6-102A, and is similar to pol CMS (pol CMS), radish cytoplasmic male sterile (Ogu CMS), tour CMS, nap CMS, etc. The types of cytoplasmic male sterility are all different, and it is a new type of cytoplasmic male sterility. The sterility and sterile line rate of the sterile line are both 100%, and it is not affected by the environment and genetic background. The cytoplasmic male sterile line of mustard mustard of the invention can be used for vegetable heterosis breeding.

Description

technical field [0001] The invention belongs to the technical field of cruciferous vegetable breeding, and in particular relates to a new breeding method for a cytoplasmic male sterile line of mustard mustard. Background technique [0002] Cytoplasmic male sterility (CMS for short, the same below) is the most important way to utilize heterosis in rapeseed. At present, there are many types of cytoplasmic male sterility reported at home and abroad. According to their different sources, they can be divided into two categories: heterologous cytoplasmic male sterility and homologous cytoplasmic male sterility: in heterologous CMS, the most useful and has begun There are ogu CMS, kosena CMS and tour CMS for commercial breeding; among homologous CMSs, the most commercially valuable is the Polima cytoplasmic male sterile line (abbreviated as Pol CMS, the same below). Pol CMS is the first international rapeseed pol CMS line with practical value discovered by Professor Fu Tingdong of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04C12N15/65
Inventor 傅廷栋万正杰马朝芝涂金星李兴华易斌文静
Owner HUAZHONG AGRI UNIV
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