Microscopically observable cone-plate shear field generator

Abstract

The invention discloses a cone-plate shear field generating device capable of microscopic observation, which includes: a base; a longitudinal driving device arranged on the base; a U-shaped bracket, the bottom of which is connected and arranged on the base On the longitudinal driving device; a liquid pan bracket, which is arranged on one side of the U-shaped bracket, and a liquid pan is arranged on the liquid pan bracket; a vertical driving device, which is arranged on the other side of the U-shaped bracket One side; a cone-plate support, the rear end of which is connected to the vertical drive device, and the lower part of the front end of the cone-plate support is provided with a cone-plate, the cone-plate is facing the liquid container, and is connected to the liquid container Coaxial; a motor, which is arranged on the cone-plate support, and the motor is connected with the cone-plate through a reduction transmission device. The structure of the invention does not affect the adjustment of the optical path of the microscope, so it can be used in conjunction with different types of microscopes, which is convenient for users.

Description

technical field [0001] The invention relates to a cone-plate shear field generating device, in particular to a microscopically observable cone-plate shear field generating device for basic research on the behavior of adherent cultured cells and suspension cells in a flow field. Background technique [0002] When the body fluid of the human body flows in the lumen, it will cause a shear field (such as blood flowing in the blood vessel). The cells in the human lumen, including suspension cells (such as blood cells) and cells growing on the wall (such as endothelial cells), in The metabolism and growth status in the shear field correspond to certain physiological and pathological characteristics. For example, red blood cells are deformed by the shear field in the process of entering the capillary, so that they can pass through the capillary smoothly; platelets can aggregate under the induction of high shear field, causing arterial thrombosis and endangering human health; The i...

AI Technical Summary

Problems solved by technology

But this device has certain disadvantages: firstly, the core part of the device, the cone-plate shearing device, uses the cone and the plate itself as the axis of rotation, and cannot be easily disassembled and cleaned by the gears around the cone. Installation is difficult to achieve the original accuracy

Adding pipes in this environment has an irreversible effect on the shear field - there is no longer a uniform shear field between the cone and plate, which is not conducive to rigorous scientific experiments

Third, due to the inconvenient disassembly and cleaning of the cone and plate, the device cannot be used to cultivate adherent cells, so it is impossible to observe the behavior of adherent cells in the shear field

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