Porphyra suspended filamentous form cell ripening accelerating device and method

A technology of filaments and cells, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem of affecting the normal operation of seedlings, difficult to naturally mature and release conchospores, and unsatisfactory Production needs and other issues, to achieve the effect of easy installation, low cost and obvious effect

Inactive Publication Date: 2005-09-28
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The collection of seaweed seedlings is like the sowing of land crops, which has a strong seasonality. It is generally difficult for the suspended filamentous cells of seaweed cultivated indoors to mature naturally and release conchospores, and only a small amount of ascospores can be produced in the seedling harvesting season. , which seriously affects the normal operation of seedling production and cannot meet the production demand

Method used

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  • Porphyra suspended filamentous form cell ripening accelerating device and method

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Effect test

Embodiment 1

[0014] Such as figure 1 As shown, the device of the present invention is composed of four parts: a flushing bag, an overflow bucket, a water pump and a water return tank. The flushing bag 5 is cylindrical, with a diameter of 30 cm and a height of 40 cm. 60cm, 80cm high plastic bucket, the mouth of the bucket is the overflow. The small submersible pump 3 is equipped with a 32mm inner diameter hose 4 to discharge water to impact the cells, with an hourly flow rate of ≥3t and a lift of ≥5m (in this embodiment, an hourly flow rate of 4t and a lift of 6m), and the model is not limited. The backwater tank is deep ≥ 40cm (the depth of the backwater tank in this embodiment is 40cm), and the area is not limited. When picking seedlings, each flushing bag 5 accommodates 150 to 450 grams of filamentous cells (300 grams in this embodiment), and is arranged in the overflow bucket 8. Inject 30cm deep clean filtered seawater into the water. The number 2 among the figure is the liquid level...

Embodiment 2

[0019] The difference from Example 1 is that it is used for the smaller-scale seedling picking test:

[0020] Its device: the overflow bucket 8 is a diameter of 40cm, a plastic bucket of high 60cm, and the mouth of the bucket is the water overflow. The small submersible pump 3 is equipped with a 32mm inner diameter hose 4 to discharge water to impact the cells, with a flow rate of 3t per hour and a return tank depth of 40cm. Each flushing bag 5 accommodates 100 grams of filamentous cells when picking seedlings, and is arranged in the overflow bucket 8, and the top of the flushing bag 5 is higher than the overflow opening 15cm, and injects 30cm deep clean filtered seawater in the backwater tank 1.

[0021] Its method: every evening (this embodiment is 20 o'clock in the afternoon) starts submersible pump to make the cells under the 5 bag bodies of the flush water bag keep the state of constantly rolling all night under the impact of the water flow, and finish flushing at 4 o'clo...

Embodiment 3

[0023] The difference from Example 1 is:

[0024] Its method: start the submersible pump every evening (this embodiment is 18 o'clock in the afternoon) to make the cells under the 5 bags of the flushing bag keep the state of constantly rolling all night under the impact of the water flow, and finish flushing at 8 o'clock in the morning. The water flow rate is controlled at 6 tons per hour. After 3 days of continuous operation, it lasts for 12 hours. The cells enter the mature release state for 3 days. The total amount of spores released per gram of cells reaches 35 million spores.

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Abstract

The present invention discloses one kind of suspending laver filiform cell mature promoting apparatus and method. In proper zoospore collecting season, the suspension cultured cells may be made to mature in great amount to produce conchospores with normal germination function for collecting. The apparatus consists of four parts including flushing bag, overflow barrel, water pump and water returning channel. After nearly mature laver filiform cell in 150-450 g is set inside the flushing bag, the water pump is made to run for 2-4 nights so that water inside the bag is rolled to make the cell become mature. The present invention can meet the requirement of raising laver spore.

Description

technical field [0001] The invention relates to laver cultivation technology, in particular to a laver suspended filamentous cell ripening device and method. Background technique [0002] The collection of seaweed seedlings is like the sowing of land crops, which has a strong seasonality. It is generally difficult for the suspended filamentous cells of seaweed cultivated indoors to mature naturally and release conchospores, and only a small amount of ascospores can be produced in the seedling collection season. , Seriously affect the normal operation of seedling production, can not meet the production demand. Contents of the invention [0003] In view of the low success rate of laver suspended filamentous cell seedling cultivation mentioned above, the object of the invention is to provide a laver suspended filamentous cell ripening device and method that can improve the success rate of seedling harvesting. [0004] In order to achieve the above object, the technical schem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 费修绠陈兰涛邹立红张京浦彭光于义德
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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