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Ginsenoside Compound-K preparing method

A technology of ginsenosides and total ginsenosides, which is applied in the field of pharmacy, can solve the problems of low CompoundK content and inability to be separated, and achieve the effects of convenient extraction and separation, benefit to the pharmaceutical or health care product industry, and high conversion rate

Inactive Publication Date: 2005-01-26
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented process involves direct use of specific compounds from Gingsma sinensis for their ability to reduce blood pressure without causing damage during surgery procedures. It also includes methods like solid state fermentations that can be used with different types of sugars while still producing desired products at reasonable costs. Overall this new technique simplifies the manufacturing processes by eliminating steps involved before extracting pure ingredients needed for production.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding new sources of natural products containing specific components called “Compounds” like those extracted directly from ginshaped root crops including Pennate Linnaceans, Ananas Tree Hoods, Boea cereals, Chinese yarnherbs, licensed medicine materials obtained during cultivating processes.

Method used

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  • Ginsenoside Compound-K preparing method
  • Ginsenoside Compound-K preparing method
  • Ginsenoside Compound-K preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Collect soil samples, dry them, pour them into a pre-sterilized mortar, grind them finely, spread them on white paper irradiated with ultraviolet light, pop them onto potato plates, and cultivate them at 28°C. Pick a single colony of mold and culture them on the slant medium for fermentation. Strain screening.

Embodiment 2

[0071] Aspergillus niger was grown on Chua's medium (28°C, 7 days). After the spores were plump, they were inserted into the seed medium containing glucose-corn steep liquor-ammonium sulfate, and rotated at 25-35°C and 200rpm. When the mycelium grows luxuriantly, the seed culture solution is put into the fermenter at a ratio of 5-10%, at 26-28°C, the speed is increased from 200rpm to 800rpm, and the dissolved oxygen is 20-40%, and cultivated for 60-72 hours. Feed 2% of total saponins in stems and leaves of Panax notoginseng, and continue culturing for 40-56 hours to transform ginsenosides. According to HPLC analysis, the fermentation conversion rate is 12%, the final extraction and separation yield is 5.25%, and the Compound K content is above 97%.

Embodiment 3

[0073] Aspergillus niger (Aspegillus niger) spore suspension was inoculated into a 5L shake flask containing 1L glucose-corn steep liquor-ammonium sulfate medium, pH 5.0, cultivated on a rotary shaker at 30°C at 180r.p.m for 72h, 1% cultivated total ginsenosides Feeding, conversion 48h. Alcohol extraction of the fermentation conversion solution, filtration, vacuum evaporation, methanol dissolution, HPLC analysis, the conversion rate is 8%, the final extraction and separation yield is 5%, and the Compound K content is more than 80%.

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Abstract

The invention relates to a method for preparing ginsenoside Compound-K, which belongs to the pharmacology field. The Compound-K is prepared by fermenting conversion with the ginseng total saponins as substrate by using fungus aerobiotic fermentation technology, immobilized cell technology, and immobilized enzyme technology, and the Compound-K is separated and purified. The content analyse and structure determination for the Compound-K are carried out. The detection results show that the structure of the product provided by the invention is coincident with the structure of the Compound-K.

Description

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Claims

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Application Information

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Owner FUDAN UNIV
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