Application of alpha-fodrin polypeptide resisting antibody determination in the diagnosis of xerosis syndromes

A technology of fodrin and protein, which is applied in the field of anti-α-fodrin polypeptide antibody detection in the diagnosis of Sjögren's syndrome, and can solve problems such as delayed diagnosis, poor medical compliance, and lost treatment opportunity

Inactive Publication Date: 2003-03-19
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no ideal serological diagnosis method has been found clinically. In addition to clinical symptoms, the diagnosis is mainly made by invasive examinations such as lip gland biopsy and parotid angiography. Due to poor medical compliance of patients, diagnosis is often delayed and the best treatment is lost. opportunity

Method used

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  • Application of alpha-fodrin polypeptide resisting antibody determination in the diagnosis of xerosis syndromes
  • Application of alpha-fodrin polypeptide resisting antibody determination in the diagnosis of xerosis syndromes
  • Application of alpha-fodrin polypeptide resisting antibody determination in the diagnosis of xerosis syndromes

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Experimental program
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Effect test

Embodiment Embodiment 1

[0024] Examples Example 1 Design and Synthesis of α-fodrin Polypeptide

[0025] The aforementioned research work (1-4) has proved that the α-fodrin fusion protein purified by genetic engineering can specifically bind to the α-fodrin antibody in patients with Sjögren’s syndrome, and then through specific methods It can be checked out. Utilize Flechsler etc. at first in the test of the present invention (5) The reported solid-phase synthesis method synthesized a α-fodrin polypeptide with a total of 20 amino acids, and its sequence is as follows:

[0026] RQKLEDSYRFQFFQRDAEEL

[0027] Arg-Gln-Lys-Leu-Glu-Asp-Ser-Tyr-Arg-Phe-Gln-Phe-Phe-Gln-Arg-Asp-Ala-Glu-Glu-Leu This sequence is nonerythroid α -spectrin) amino segment sequence of α-fodrin.

[0028] Studies have shown that the above-mentioned α-fodrin polypeptide can specifically recognize the specific antibody in the serum of patients with Sjögren's syndrome, which is of great significance for the diagnosi...

Embodiment 2

[0028] Studies have shown that the above-mentioned α-fodrin polypeptide can specifically recognize the specific antibody in the serum of patients with Sjögren's syndrome, which is of great significance for the diagnosis of Sjogren's syndrome. Example 2 Detection of α-fodrin polypeptide in the serum of patients with Sjogren's syndrome

[0029] In this study, enzyme-linked immunosorbent assay (ELISA) was used to detect the anti-α-fodrin polypeptide antibody in the serum of patients with Sjogren's syndrome, rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) to evaluate The distribution of the polypeptide antibody in various diseases.

[0030]First, the α-fodrin polypeptide is coated on an ELISA microwell plate and incubated with the serum to be tested. If there is an anti-α-fodrin polypeptide antibody in the serum, the antibody will bind to the coated solid. Combine with antigen to form antigen-antibody complex. After washing the unbound antibody and other compon...

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Abstract

The present invention provides one kind of alpha-fodrin polypeptide and its analog used for the detection of xerosis syndromes. This kind of polypeptide antigen may be combined specifically with the specific antibody in the body of xerosis syndromes patient and has very high sensitivity and specificity.

Description

[0001] The invention relates to the preparation of α-fodrin polypeptide and its application as an antigen in detecting Sjögren's syndrome and other diseases. technical background [0002] Sjogren's syndrome (SS) is a systemic autoimmune disease characterized by invading exocrine glands such as lacrimal glands and salivary glands. The most common clinical manifestations of the disease are dry eyes and dry mouth, and can involve the lungs, kidneys, lymphatic system, blood vessels and liver and other organs. The disease has no obvious regional distribution difference, and can occur at any age, with 40-60 years old, especially postmenopausal women in the majority, and the ratio of male to female is 1:9. The prevalence rate in my country is 0.77%, and the prevalence rate in the elderly population is as high as 4%. There are nearly 10 million patients in the whole country. Sjogren's syndrome can be divided into two types: primary Sjogren's syndrome (Primary Sjogren's syndrome) and ...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/68
Inventor 栗占国
Owner PEOPLES HOSPITAL PEKING UNIV
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