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Enzyme-linked immunosorbent assay kit for diagnosis of testicular trematodiasis, paragonimiasis, pork-measles disease and sparganosis

A clonorchis sinensis and kit technology, which is applied in the field of enzyme-linked immunosorbent assay kits

Inactive Publication Date: 2002-12-11
SHIN POONG PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present inventors have conducted extensive research to solve previous problems in the diagnosis of parasites in tissues

Method used

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  • Enzyme-linked immunosorbent assay kit for diagnosis of testicular trematodiasis, paragonimiasis, pork-measles disease and sparganosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of Antigen Covering Small Plates (1) Preparation of Antigens of Clonorchis sinensis, Paragonimus Westermannus, Cysticercus suis and Sparganum

[0039] Metacercariae of Clonorchis sinensis and Paragonimus westmannii were isolated from naturally infected intermediate hosts (Clonorchis sinensis: Pseudorasbora parva, Paragonimus westmansi: Cambaroides similis). Rabbits and dogs were orally infected with Clonorchis sinensis and Paragonimia westermannii, respectively. After 3 to 4 months, 2 g or more of adults of Clonorchis sinensis and Paragonimus westmannii, respectively, were obtained from these animals.

[0040] Larvae of Taenia solium were isolated from pigs naturally infected with Cysticercus suis in endemic areas, and cyst fluid was isolated from larval cysts. About 2 g or more of spargan larvae were isolated from naturally infected snakes.

[0041] Obtained Clonorchis sinensis and Paragonimus westmannii adults and spargania were washed with 10...

Embodiment 2

[0047] Embodiment 2: the preparation of negative standard serum

[0048] 10 ml of blood were collected from persons showing no reactivity to the Clonorchis sinensis, Paragonimus westermannii, Cysticercus suis and Sparganum antigens and the serum was separated from the blood. Serum was inactivated by heating to 56°C for 30 minutes. Filter using a sterile filter. Quantified at 1000μl, add 0.5μl Proclin300 to obtain 1ml negative standard serum. Serum was aliquoted into 15 μl vials and refrigerated until use.

Embodiment 3

[0049] Embodiment 3: the preparation of positive standard serum

[0050] (1) positive control serum of clonorchiasis

[0051] 10 ml of blood were collected from persons confirmed to contain Clonorchis sinensis eggs by stool testing, and serum was separated from the blood. Serum was inactivated by heating to 56°C for 30 minutes. Filter using a sterile filter. Quantitatively at 1000μl, add 0.5μl Proclin300 to obtain lml Clonorchis sinensis positive standard serum. Serum was aliquoted into 5 μl vials and refrigerated. Serum was used after dilution (4 μl serum per 100 μl buffer).

[0052] (2) Positive control serum for paragonimiasis

[0053] Serum was separated from blood collected from 10 ml of blood collected from persons confirmed to be infected with Paragonia westermannii or reactive with specific antigens. Serum was inactivated by heating to 56°C for 30 minutes. Filter using a sterile filter. Quantified in 1000 μl, 0.5 μl Proclin300 was added to obtain 1 ml Westerman...

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Abstract

The invention discloses an ELISA kit, which can simultaneously diagnose the infection of Clonorchis sinensis, Paragonimus westermannii, cysticercosis and sparganosis. The kit provided by the invention can simply, quickly and accurately diagnose the infection of the main tissue parasitic helminth parasites at one time through a standardized method.

Description

technical field [0001] The present invention relates to the serological diagnosis of tissue parasitic infections. More specifically, the present invention relates to an enzyme-linked immunosorbent assay (hereinafter abbreviated as ELISA) kit, which can simultaneously diagnose Clonorchis sinensis, Paragonimus westernmani, Cysticercus cellulosae) and Sparganum (Sparganum). Background technique [0002] In Korea, human intestinal parasites such as Ascaris hominis, Trichocephala, Hookworm and Trichostrongylus orientalis (which are often detected by stool tests) have been significantly reduced and are therefore now negligible. Currently, such stool tests are only performed in medical institutions such as hospitals. On the contrary, hitherto, tissue-dwelling parasites continue to emerge and, therefore, become relatively more important. Furthermore, tissue-dwelling parasites cause more severe damage than intestinal parasites, and therefore, their infection...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/569G01N33/96
CPCG01N33/56966G01N33/96
Inventor 赵昇烈蔡鍾一洪性臺韩信赵一焕
Owner SHIN POONG PHARMA CO LTD
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