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Duplication method of chmice acute hyperuricemia model

A technology of hyperuricemia and replication methods, which is applied in the field of replication of acute hyperuricemia models in mice, can solve the problems of not reaching the level of urate deposition and not being able to form gouty arthritis

Inactive Publication Date: 2007-05-09
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(James J, Carroll, Holly Coburn, et al.Asimplified alkaline phosphotungstate assay for uric acid insulin[J].Clinical Chemistry, 1997,17(3):158~160), but the high uric acid level caused by oxonic acid has not been If it exceeds 7mg / dl, although it can be maintained for 5 hours, it cannot reach the level of urate deposition, so it cannot form gouty arthritis
Using the genetic mutation method to replicate animal models, this method makes more than half of the mutant mice unable to survive for more than 4 weeks, and the literature has no conclusions about this method, especially the difficulty of this model

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The replication method of embodiment 1. mouse acute hyperuricemia model, its method is as follows:

[0028] Select Kunming mice, male, 20g±2g, at room temperature, by intragastric administration, and infuse hypoxanthine 1000mg·kg -1 , by intraperitoneal injection, inject OA 100mg·kg -1 .

Embodiment 2

[0029] The replication method of embodiment 2. mouse acute hyperuricemia model, its method is as follows:

[0030] Select Kunming mice, male, 20g±2g, at room temperature, by intragastric administration, and infuse hypoxanthine 1000mg·kg -1 , by intraperitoneal injection, inject OA 600mg·kg -1 .

Embodiment 3

[0031] The replication method of embodiment 3. mouse acute hyperuricemia model, its method is as follows:

[0032] Select Kunming mice, male, 20g±2g, at room temperature, by intragastric administration, and infuse hypoxanthine 1000mg·kg -1 , by subcutaneous injection, inject OA 100mg·kg -1 .

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Abstract

The invention discloses a duplication method of chmice acute hyperuricemia model which comprises selecting ICR healthy female or mouse of Kunming specie with a body weight of 16g-35g under room temperature, administering the rats simultaneously by purines, namely hypoxanthine or dioxopurine by 100-1500mg / kg and uric acid depressant or allantoxanamide through stomach filling, hypodermic injection or abdominal cavity injection. Experimental investigation has shown that by using uric acid enzyme inhibitor in combination with purines, stabilized mouse hyperuricemia model can be obtained within 1 hour.

Description

1. Technical field [0001] The invention relates to a replication method of an animal model, in particular to a replication method of a mouse acute hyperuricemia model. 2. Background technology [0002] Gout is a group of heterogeneous diseases caused by purine metabolic disorders or (and) decreased uric acid excretion. Clinically, hyperuricemia is the main feature. Hyperuricemia is the biochemical basis of gout, and gout must be accompanied by hyperuricemia. Essentially, there is no strict boundary between hyperuricemia and gout. In recent years, research on the pathogenesis of gout and its drug treatment has become one of the hotspots in the medical field. At present, there is still a lack of mature animal models of gout at home and abroad, and the establishment of animal models of hyperuricemia is a prerequisite for gout research. There are still many imperfections in the existing models, which brings great inconvenience to the research of gout. The following is a brief...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 徐立时乐
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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