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Method for counting leukocytes and leukocyte counter

A technique for counting white blood cells and total white blood cells, applied in the field of counting devices

Inactive Publication Date: 2004-11-03
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although these counting methods are different according to human vision or different measurement results of instruments, it is a method for actually counting the number of blood cells.

Method used

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  • Method for counting leukocytes and leukocyte counter
  • Method for counting leukocytes and leukocyte counter

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Embodiment Construction

[0009] There are five types of white blood cells: neutrophils, eosinophils, basophils, monocytes, and lymphocytes. The above three types of neutrophils, eosinophils, and basophils are collectively called Granulocytes. Moreover, the number of leukocytes in the blood and the ratio of each fraction are slightly different in children and the elderly, but there is no gender difference and almost maintains a constant value. For example, the number of white blood cells in adult blood is about 6700 / μl, and the average ratio of its fractions is 55.3% for neutrophils, 3.5% for eosinophils, 0.5% for basophils, and 5.0% for monocytes , Lymphocytes 36.6%. In addition, granulocytes are the most numerous among white blood cells, and there is a large amount of elastase inside them. Granulocyte elastase is a substance released when granulocytes are stimulated or damaged by phagocytes and inflammation, and thus correspond to lesions. In the blood, the above-mentioned elastase is in an inacti...

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Abstract

A method for counting leukocytes which comprises liberating elastase from granulocytes contained in a specimen, adding an anti-granulocyte elastase antibody to the thus liberated elastase, measuring the antibody bonded to the elastase to thereby determine the concentration of the elastase, and then calculating therefrom the number of leukocytes contained in the specimen with the use of the known ratio of the leukocyte count to the elastase concentration. This method makes it possible to conveniently and less expensively count leukocytes at a high accuracy comparable to the one established by using a conventional automatic blood cell counter, as the figure shows.

Description

technical field [0001] The invention relates to a method for counting white blood cells in a sample and a counting device used in the counting method. Background technique [0002] In blood tests, white blood cell counts are generally performed, which is of great clinical significance. For example, the presence or degree of anemia can be checked by the red blood cell count, and various symptoms caused by tissue hypoxia can be diagnosed. On the other hand, white blood cells are the core of the body's defense function, that is, immune function, which can resist bacteria and viruses that invade the body. In particular, granulocytes and leukocytes have the function of releasing proteolytic enzymes such as elastase to act when bacteria and foreign proteins invade the body. In many diseases, the number of white blood cells increases or decreases. Therefore, the white blood cell count is very important for the screening of diseases, and it becomes a very important inspection item...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49G01N33/50G01N33/53G01N33/543G01N33/573
CPCG01N2333/966G01N33/573G01N33/53
Inventor 大久保章男山田繁树
Owner ARKRAY INC
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