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Grease degrading bacterium as well as screening method and application thereof

A screening method, a technology for degrading bacteria, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of poor growth ability, low activity, difficult to degrade oil, etc., to achieve simple nutritional requirements, Strong environmental adaptability and cost reduction effect

Pending Publication Date: 2022-04-29
ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, general oil-degrading bacteria have poor growth ability in medium and low temperature conditions, so the ac

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  • Grease degrading bacterium as well as screening method and application thereof
  • Grease degrading bacterium as well as screening method and application thereof
  • Grease degrading bacterium as well as screening method and application thereof

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[0025] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] Culture medium preparation

[0027] Enrichment medium: peptone 10.0g, yeast powder 5.0g, NaCl 5.0g, soybean oil 20.0g, deionized water to 1000mL, pH natural, sterilized at 121°C for 20min.

[0028] LB medium: peptone 10g, yeast powder 5.0g, NaCl 10.0g, deionized water to 1000mL, pH natural, sterilized at 121°C for 20min.

[0029] Tributyrin solid medium: special peptone 5.0g / L, yeast extract 3.0g / L, agar 20.0g / L, tributyrin 10.0g / L, deionized water to 1000mL, pH natural .

[0030] Inorganic salt medium / rescreening medium: (NH 4 ) 2 SO 4 5.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, NaCl 2.0g, NaH 2 PO 4 2.0g, 10.0g soybean oil, dilute to 1000mL with deionized water, natural pH, sterilize at 121°C for 20min.

[0031] Fat seed medium: glucose 20.0g, peptone 20.0g, (NH 4 ) 2 SO 4 5.0g, K 2 HPO 4 1.0g, Mg...

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Abstract

The invention discloses a grease degrading bacterium, the classification name of the strain is Microbacterium esteraromatum, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.22787, and the invention also discloses a screening method and application of the strain. The JY34 obtained by the invention can well grow under a medium temperature condition, and the lipase enzyme activity reaches 65 U/mL to 160 U/mL; the method is simple in process, high in environmental adaptability and simple in nutritional requirement, and can efficiently degrade grease in edible soybean oil and kitchen waste wastewater, and the grease degradation rate reaches about 70%-80%.

Description

technical field [0001] The invention relates to the technical field of kitchen waste treatment, in particular to a grease-degrading bacteria and its screening method and application. Background technique [0002] The treatment methods of food waste mainly include landfill, incineration, anaerobic fermentation, aerobic composting, feed, etc. However, the water content of food waste is as high as 70%-90%, and the oil content on a wet basis is as high as 20%-30%. In the early stage of each treatment method, the food waste needs to be filtered and cleaned to varying degrees, thereby producing a large amount of oily wastewater . Animal and vegetable oils and fats are multi-component alkyl fatty acids with high biological oxygen demand (BOD) and chemical oxygen demand (COD). Specifically, restaurant oily wastewater contains high concentrations of fats, oils, and greases and large amounts of suspended solids, leading to the occurrence of malodorous and clogged effluents (Brooksba...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01
CPCC12N1/20C12N1/02C02F3/34
Inventor 李季丁国春朱廷恒易蒲红李芩萍赵彩虹丁晓艳徐志伟林路成
Owner ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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