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Anthurium andraeanum tissue culture and rapid propagation culture medium and tissue culture and rapid propagation seed production method

The technology of a culture medium and anthurium group, which is applied in the field of plant tissue culture, can solve the problems of not being able to achieve fast propagation and taking a long time, and achieve the effects of improving the success rate, reducing the mutation rate and reducing the pollution rate.

Pending Publication Date: 2022-04-08
新疆维吾尔自治区阿克苏地区林业技术推广服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in S2 and S3, it takes a long time and cannot achieve the purpose of rapid propagation

Method used

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  • Anthurium andraeanum tissue culture and rapid propagation culture medium and tissue culture and rapid propagation seed production method
  • Anthurium andraeanum tissue culture and rapid propagation culture medium and tissue culture and rapid propagation seed production method
  • Anthurium andraeanum tissue culture and rapid propagation culture medium and tissue culture and rapid propagation seed production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0033] Embodiment 1-3 result

[0034]

[0035]

[0036] 3) Proliferation of callus: Inoculate the callus into the callus proliferation medium, and culture it for 20-25 days at a temperature of 28°C, a light intensity of 2100-2300Lux, and a light of 12h / d to obtain a 1cm×1cm Large and small callus with adventitious bud points;

[0037] Embodiment 1-3 result

[0038]

[0039] 4) Callus differentiation culture: inoculate the callus into the differentiation medium, and culture it for 30-35 days under the conditions of temperature 28°C, light intensity: 3200-3300Lux, light 15h / d, and the adventitious buds on the callus will transform into seedlings ;

[0040] Embodiment 1-3 result

[0041]

[0042]

[0043] 5) Subculture: Inoculate the callus that has grown seedlings into the subculture medium, and culture for 30 days under the conditions of temperature 28°C, light intensity 3200-3300Lux, and light 15h / d, and the callus grows into bands Adventitious buds, and compl...

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Abstract

The invention discloses a culture medium for tissue culture and rapid propagation of anthurium andraeanum. The culture medium comprises a callus induction culture medium, a callus proliferation culture medium, a callus differentiation culture medium, a subculture medium and a rooting culture medium, the anthurium andraeanum tissue culture and rapid propagation seed production method comprises the following steps: (1) preparing explants; (2) inducing calluses; (3) propagating the calluses; (4) differentiating and culturing the calluses; by utilizing the sterilization method disclosed by the invention, the pollution rate is effectively reduced, the culture medium formula is utilized, the callus induction rate is high, the callus differentiation variation is low, the culture period is greatly shortened, the probability of variation seedlings during subculture is reduced, the generations of subculture are prolonged, and the yield is increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to an anthurium tissue culture rapid propagation medium and a tissue culture rapid propagation seed production method. Background technique [0002] Anthurium andraeanum belongs to Araceae Anthurium, also known as Anzuhua and Anthurium, and is a perennial herbaceous flower. Popular precious flowers. Anthurium has become the main variety in the flower market at present, and the market demand is large, and the traditional breeding method can no longer meet the market demand. Therefore the adoption of tissue culture rapid propagation technology is an effective way for a large number of anthuriums to propagate seedlings quickly and uniformly. Anthurium tissue culture rapid propagation is prone to undifferentiated callus, weak growth of tissue culture seedlings, and high mutation rate. [0003] In addition, using Anthurium stem tips as explants has more bacteria in the bo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 赵英赵一卓徐航张志刚韩晓燕郑新国孔德智
Owner 新疆维吾尔自治区阿克苏地区林业技术推广服务中心
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