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Application of compound cyproheptadine hydrochloride in preparation of medicine for preventing or treating African swine fever

A technology for cyproheptadine hydrochloride and African swine fever, which is used in the prevention or treatment of African swine fever. The compound cyproheptadine hydrochloride can solve problems such as no research showing that Cyproheptadinehydrochloride has anti-African swine fever virus effects, and prevent the virus from invading host cells. , the effect of reducing the level of transcription and protein expression

Active Publication Date: 2022-03-15
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Okuma Hirohisa and other researchers have reported this compound in academic articles (see Okuma Hirohisa, Iijima Kazuyuki, Yasuda Takashi et al. Preventive effect of cyproheptadine hydrochloride in refractory patients with frequent migraine. [J]. Springerplus, 2013, 2:573.) , but there is no research showing that Cyproheptadinehydrochloride has anti-ASF virus effect

Method used

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  • Application of compound cyproheptadine hydrochloride in preparation of medicine for preventing or treating African swine fever
  • Application of compound cyproheptadine hydrochloride in preparation of medicine for preventing or treating African swine fever
  • Application of compound cyproheptadine hydrochloride in preparation of medicine for preventing or treating African swine fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The effect of compound Cyproheptadine hydrochloride on the replication of African swine fever virus and the transcriptional expression of genes

[0038] 1. Observe the effect of compound Cyproheptadine hydrochloride on ASFV infection and replication under fluorescence

[0039] Porcine alveolar macrophages (PAM, 2 × 10 6 Cyproheptadinehydrochloride (12μM, 18μM, 22μM, 50μM) of different concentrations dissolved in DMSO (2 Fluorescence changes were observed under a microscope after 48 hours of conditional culture.

[0040] Experimental results such as figure 1 as shown, figure 1 A shows that there is no significant difference between the infection control group and the infection blank group under fluorescent light and white light, indicating that DMSO has no effect on the infection and replication of cells.

[0041] figure 1 B shows that the fluorescence in the infection control group (DMSO) is brighter, but the fluorescence gradually becomes darker after tre...

Embodiment 2

[0068] The cytotoxicity of embodiment 2 compound Cyproheptadine hydrochloride

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Abstract

The invention belongs to the technical field of African swine fever treatment, and particularly relates to application of a compound cyproheptadine hydrochloride in preventing or treating African swine fever. The invention finds that the compound cyproheptadine hydrochloride can significantly inhibit ASFV replication. It is found that cyproheptadine hydrochloride not only inhibits transcription and protein expression of a target protein D1133L, but also can reduce transcription and protein expression levels of p30 and p72 and prevent viruses from invading host cells, and can be used for inhibiting early and late stage infection of ASFV. Therefore, the compound cyproheptadine hydrochloride can be used for preventing or treating African swine fever.

Description

technical field [0001] The invention belongs to the technical field of treating African swine fever, and in particular relates to a new application of a compound, Cyproheptadine hydrochloride, for preventing or treating African swine fever. Background technique [0002] African swine fever (ASF) is an acute, highly contagious disease caused by African swine fever virus (ASFV) infection, with a mortality rate of up to 100%. Since it was introduced into China in 2018, it has caused significant economic losses to the domestic pig industry. There is currently no commercial vaccine, so the development of effective antiviral drugs at a reasonable cost is of great importance for the treatment of ASF. Although various types of active anti-ASFV drugs have been reported, the in vivo efficacy of these compounds has not been evaluated. [0003] ASFV is a double-stranded DNA virus that replicates mainly in monocytes and macrophages and encodes more than 50 structural proteins and more ...

Claims

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Application Information

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IPC IPC(8): A61K31/4418A61P31/20
CPCA61K31/4418A61P31/20Y02A50/30
Inventor 张克山郑海学崔卉梅田宏朱紫祥冯涛杨帆曹伟军马旭升茹毅郭建宏刘湘涛
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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