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Method for co-producing ellagic acid and biological feed through microbial community fermented pomegranate rind

A technology of microbial community and biological feed, which is applied in the field of co-production of ellagic acid and biological feed by microbial community fermentation of pomegranate peel, can solve the problems of pollution extraction rate, long fermentation time, high recovery and purification cost, and achieve short fermentation time and low raw material cost. The effect of low and high nutritional value

Active Publication Date: 2022-03-01
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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Problems solved by technology

[0005] In view of the problems of high recovery and purification cost and pollution in the chemical extraction method of ellagic acid in the prior art, as well as the low extraction rate and long fermentation time of the biological fermentation method for extracting ellagic acid, the present invention proposes a co-production of pomegranate peels fermented by microbial communities Method for ellagic acid and feed, and qualitative assessment of the feasibility of residual fermented pomegranate peel residues as feed

Method used

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  • Method for co-producing ellagic acid and biological feed through microbial community fermented pomegranate rind
  • Method for co-producing ellagic acid and biological feed through microbial community fermented pomegranate rind
  • Method for co-producing ellagic acid and biological feed through microbial community fermented pomegranate rind

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Embodiment 1

[0029] A method for the co-production of ellagic acid and biological feed by microbial community fermentation of pomegranate peel:

[0030] Weigh 200 g of raw pomegranate peel (moisture content: 8.65%, cellulose content 17.38%, hemicellulose content 9.29%, raw material DPPH clearance rate 91.40%), add 200 g of tap water, and insert synthetic microbial community seed solution (inoculum size 0.5×10 7 per g of raw material), under anaerobic conditions, microbial solid-state fermentation was carried out at room temperature for 24 h (cellulose content 16.24%, hemicellulose content 7.16%). Add 1500 g of water to the fermented pomegranate peel, stir and react at 60 °C for 30 min, filter through a filter, and air-dry the filter cake at 80 °C for 12 h to obtain 136 g of biological feed (the DPPH clearance rate of the product is 97.68%). Separation and extraction of non-ionic macroporous resin Amberlite XAD-16, using distilled water as eluent, ethanol as eluent, and freeze-drying to ob...

Embodiment 2

[0032]A method for the co-production of ellagic acid and biological feed by microbial community fermentation of pomegranate peel:

[0033] Weigh 200 g of pomegranate peel raw material (moisture content: 8.65%, cellulose content 17.38%, hemicellulose content 9.29%, raw material DPPH clearance rate 91.40%), add 200 g of tap water, and access the microbial community seed solution (the inoculum size is 0.5×10 7 per g raw material), under anaerobic conditions, microbial solid-state fermentation was carried out at room temperature for 72 h (cellulose content 15.50%, hemicellulose content 7.10%). Add 1500 g of water to the fermented pomegranate peel, stir and react at 60 °C for 30 min, filter through a filter, and air-dry the filter cake at 80 °C for 12 h to obtain 123 g of biological feed (the product DPPH clearance rate is 89.89%). Separation and extraction of non-ionic macroporous resin Amberlite XAD-16, using distilled water as eluent, ethanol as eluent, and freeze-drying to obt...

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Abstract

The invention belongs to the technical field of medicines, health-care products and feed processing, and particularly relates to a method for co-producing ellagic acid and a biological feed by fermenting pomegranate rind through microbial communities. According to the method, the pomegranate rind is pretreated by adopting an anaerobic solid-state fermentation technology, cellulose and hemicellulose are degraded, the compact structure of the pomegranate rind is opened to enrich and extract the ellagic acid, the extraction rate of the ellagic acid is improved, and the method has the advantages of low cost, simple process and no pollution in the preparation process; meanwhile, the pomegranate rind filter cake is dried at low temperature to obtain the biological feed, so that the obtained biological feed has relatively strong oxidation resistance on one hand, is beneficial to digestion, absorption and utilization of animals after being subjected to microbial fermentation on the other hand, and can generate lactic acid flavor substances, so that the biological feed becomes sour and fragrant, the palatability of the animals is enhanced, and the additional value of agricultural products is improved; and certain economic benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of medicines and health products and the technical field of feed processing, in particular to a method for microbial community fermentation of pomegranate peels to co-produce ellagic acid and biological feed. Background technique [0002] my country has a long history of pomegranate cultivation. After a long period of natural selection and artificial domestication, different ecological cultivation groups have been formed with rich germplasm resources. For example, Heyin pomegranate in Xingyang City, Henan Province is a product of China's national geographical indication. It has a history of more than 2,100 years. history. Xichuan County, Henan Province developed and planted more than 50,000 mu of soft-seeded pomegranate according to local conditions, and built 8 bases of more than 1,000 mu. Pomegranate has high nutritional value and high medicinal value, and is very popular among people. At present, the inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12P17/18C07D493/06C12R1/07C12R1/225C12R1/245
CPCC12P1/04C12P17/181C07D493/06Y02P60/87
Inventor 杨旭魏涛宋丽丽张志平张靖楠马歌丽孟祥玉史志远李宁博
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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