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Inductest for triploid of Eriocheir sinensia

A Chinese mitten crab, triploid technology, applied in fish farming, application, climate change adaptation, etc., can solve the biological characteristics of limited reproduction, difficult to overcome the difficulties of in vitro culture, embryo survival rate and hatching rate Difficult to scale production and other problems, to achieve the effect of extending the effective time period, improving the operability, and increasing the doubling rate

Inactive Publication Date: 2004-03-10
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

The disadvantage of this method is that it is limited by the reproductive biological characteristics of Chinese mitten crab (river crab) brood hatching, and it is difficult to overcome the difficulty of in vitro culture. The research on crab triploid induction is limited to the embryonic stage, and it is impossibl

Method used

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Examples

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Example Embodiment

[0006] According to the reproductive biological characteristics of Chinese mitten crab and the principle of reproductive development, and in view of the main problems existing in the existing induction methods, this example changes artificial insemination to natural fertilization, and the treated eggs are naturally produced by the mother of Chinese mitten crab. The fertilized eggs are incubated together with the mother at a lower temperature, that is, the whole egg crab is used as the treatment object, and 6-DMAP is used as an inducer for treatment. Finally, triploid larvae are obtained by inhibiting the discharge of the second polar body of the fertilized egg.

[0007] The specific method of changing artificial insemination to natural fertilization to obtain fertilized eggs is: put male and female crabs into circular plastic tanks used for mating to promote production, and the temperature is constant at 12 ℃, and then the male and female crabs are combined in the same tank at a r...

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Abstract

The present invention relates to an induction method of triploid of Eriocheir sinensia, and is characterized by that in said invention, incubates the fertilizer egg and mother body are incubated together, and cytochalasin B or heat shock is utilized to make treatment or 6-DMAP is used to make treatment, finally inhibits the discharge of second polocyte of fertilized egg so as to obtain triploid larva. The described fertilizer egg is naturally produced by mother body of Eriocheir sinensia. Said invention possesses the following advantages: it can obtain fertilizer egg in a large scale, can greatly raise multiplying factor of triploid and can triploid induction rate stabilized in 60-80%.

Description

technical field [0001] The invention relates to an aquaculture method, in particular to a method for inducing triploids of Chinese mitten crabs. Background technique [0002] In the prior art, the methods for inducing triploids in fish and shellfish have been studied more, and some species such as Pacific oyster have entered the stage of large-scale application. The existing method for inducing triploid of Eriocheir sinensis generally first takes out the ovum from the mother's ovary, takes out the sperm from the seminal vesicle, and then carries out artificial insemination, and the fertilized egg of gained is hatched at 20 ℃, after hatching 15 minutes, Treat with drugs or temperature or with cytochalasin B (C.B) solution with a concentration of 1.5 mg / L, and finally obtain triploid embryos by inhibiting the discharge of the first polar body. The treatment duration is 1.5 minutes, the treatment temperature is 40° C., and the highest induction rate of triploid is 50% as detec...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 相建海崔朝霞周岭华蔡难儿
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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