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Method for low-temperature preservation of Telenomus remus Nixon

A technology for cryopreservation and noctuid moth, which is applied in the field of insect breeding, and can solve the problems of lack of low-temperature storage of noctuid moth and black egg bee, etc.

Pending Publication Date: 2021-11-05
YUNNAN TOBACCO CO LTD KUNMING BRANCH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, researchers have only conducted preliminary studies on the pupae and adults of Noctua spp., and there are still few reports on the low-temperature storage of Noctua spp.

Method used

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  • Method for low-temperature preservation of Telenomus remus Nixon

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1 A kind of method for cryopreservation of Noctuid spp.

[0027] (1) Fresh eggs of Spodoptera litura were parasitized by the female wasps of Spodoptera litura for 24 hours at the ratio of eggs of 1:50, and then the eggs that had been parasitized by Spodoptera litura were placed at 25°C, RH 75%, light Cultured under the condition of cycle 14L:10D to 1 day old;

[0028] (2) Place 1-day-old parasitic eggs at 15°C for 1 day, and then place them at 13°C for 1 day for low-temperature acclimatization;

[0029] (3) After the low-temperature acclimation is completed, lower the ambient temperature to 5°C at a cooling rate of 3°C / h, refrigerate the parasitic eggs for 6 days, and then place the parasitic eggs at 26°C, RH 75%, and photoperiod 14L:10D to continue Cultivate until the moth black egg bee emerges.

Embodiment 2

[0031] (1) The fresh eggs of Spodoptera litura were parasitized by the female wasp of Spodoptera litura for 24 hours at the ratio of wasp eggs of 1:50, and then the eggs that had been parasitized by Spodoptera litura were placed at 26°C, RH 70%, light Under the condition of cycle 15L:9D, culture to 6 days old;

[0032] (2) Place 6-day-old parasitic eggs at 16°C for 0.5 days, and then place them at 12°C for another 0.6 days for low-temperature acclimatization;

[0033] (3) After the low-temperature acclimation is completed, reduce the ambient temperature to 8°C at a cooling rate of 4°C / h, refrigerate the parasitic eggs for 9 days, and then place the parasitic eggs at 27°C, RH 65%, and photoperiod 15L:9D to continue Cultivate until the moth black egg bee emerges.

Embodiment 3

[0035] (1) Fresh eggs of Spodoptera litura were parasitized by the female wasp of Spodoptera litura for 24 hours at the ratio of wasp eggs of 1:50, and then the eggs that had been parasitized by Spodoptera litura were placed at 27°C, RH 65%, light Cultured to 9 days old under the condition of cycle 14L:9D;

[0036] (2) Place 9-day-old parasitic eggs at 16°C for 0.7 days, and then place them at 12°C for 0.8 days for low-temperature acclimation;

[0037] (3) After the low-temperature acclimation is completed, lower the ambient temperature to 10°C at a cooling rate of 3°C / h, refrigerate the parasitic eggs for 3 days, and then place the parasitic eggs at 25°C, RH 70%, and photoperiod 14L:10D to continue Cultivate until the moth black egg bee emerges.

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Abstract

The invention discloses a method for low-temperature preservation of Telenomus remus Nixon, and belongs to the technical field of insect breeding. The method specifically comprises the following steps: firstly, culturing parasitic eggs after parasitism of Telenomus remus Nixon to 1-9 days old, then carrying out two-step low-temperature domestication on the cultured parasitic eggs, after the low-temperature domestication is completed, reducing the environment temperature to 5-10 DEG C, and then refrigerating the parasitic eggs for 3-9 days. The Telenomus remus Nixon preserved at low temperature can achieve the eclosion rate and the parasitic rate which are basically equivalent to those of an unrefrigerated control group, the individual size is not affected by low-temperature preservation, and the method has great positive significance in large-scale breeding, transportation and biological control of the Telenomus remus Nixon.

Description

technical field [0001] The invention relates to the technical field of insect breeding, in particular to a method for cryopreservation of the noctuid moth bee. Background technique [0002] Telenomus remus Nixon belongs to the family Platygastridae of the order Hymenoptera and is an important parasitic natural enemy in the egg stage of various Lepidoptera Noctuidae insects. In 1937, Nixon first discovered and described the wasp in the eggs of Noctuid genus collected in Malaysia. In 2007, Shen Zhirong reported in the research on the biological control of Spodoptera litura that S. litura is a natural enemy insect. Then Xu Zaifu et al. reported on the ontogeny of S. litura. In 2019, Huo Liangxiao et al. found that the parasitism rate of S. litura and Spodoptera frugiperda eggs accumulated in multiple layers was over 90%; Controlling the eggs of Spodoptera frugiperda, it was found that the field parasitism rate of Spodoptera frugiperda was over 80%. Zhu Kaihui and others also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 谢永辉蔺忠龙王志江杨明陈雅琼戴恩张永贵许东亚李梦娇吴志美朱涛吴国星
Owner YUNNAN TOBACCO CO LTD KUNMING BRANCH
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