Pueraria, scutellaria baicalensis and coptis extract, preparation method, pharmaceutical preparation and application
A technology of pueraria chinensis and pharmaceutical preparations, applied in the field of pueraria chinensis extracts, can solve problems such as potential safety hazards, and achieve the effects of good safety, short time consumption and good reproducibility
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Embodiment 1
[0046] The preparation method of the Pueraria chinensis extract of the present embodiment comprises the following steps:
[0047] Step 1): Take Pueraria lobata, Scutellaria baicalensis, Coptis chinensis and Zhigancao according to the weight ratio of 8:3:3:2, heat and reflux with water to extract twice, and the amount of water added each time is 8 times and 6 times the total weight of the above raw materials respectively, each time The extraction time was 1.5 hours and 1 hour, respectively, and the two extracts were combined to obtain the total extract after filtration. Concentrate the total extract under reduced pressure below 85°C, recover the solvent, and obtain the primary extract of Pueraria baicalensis.
[0048] Step 2): use preparative liquid chromatography to remove berberine in the primary extract of Pueraria chinensis. Preparative liquid chromatography with C 18 Silica gel was used as filler (Agilent Prep-C18, 21.2mm×250mm, 5 μm), 0.1% formic acid-water was used as ...
Embodiment 2
[0053] Get the first extract of Pueraria chinensis prepared in step 1) of Example 1, filter and go to C 18 Silica gel preparative liquid chromatography (Agilent Prep-C18, 21.2mm × 250mm, 5μm), with methanol as mobile phase A, water as mobile phase B, gradient elution (0 ~ 60min, 10% ~ 100% A), the flow rate is 10ml / min, the column temperature is room temperature, and an ultraviolet detector is used for detection, and the detection wavelength is 346nm. Determine the resolution of berberine from adjacent chromatographic peaks.
Embodiment 3
[0055] Get the first extract of Pueraria chinensis prepared in step 1) of Example 1, filter and go to C 18 Silica gel preparative liquid chromatography (Agilent Prep-C18, 21.2mm × 250mm, 5 μ m), with ethanol as mobile phase A, water as mobile phase B, gradient elution (0 ~ 60min, 10% ~ 100% A), the flow rate is 10ml / min, the column temperature is room temperature, and an ultraviolet detector is used for detection, and the detection wavelength is 346nm. Determine the resolution of berberine from adjacent chromatographic peaks.
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