Method for identifying less than 25 pieces of pseudo-ginseng and less than 80 pieces of pseudo-ginseng based on non-targeted metabonomics

Abstract

The invention provides a method for identifying 25 or less pseudo-ginseng and 80 or less pseudo-ginseng based on non-targeted metabonomics, and belongs to the field of analysis and detection of traditional Chinese medicinal materials, the method comprises the following steps: S1, extracting a pseudo-ginseng metabolite by using a methanol solution as a solvent to obtain a test sample; S2, carrying out non-targeted component determination on the pseudo-ginseng metabolite extracting solution by adopting a UHPLC LTQ Oribitrap MS (Ultra High Performance Liquid Chromatography LTQ Oribitrap Mass Spectrometry) combined technology to obtain original data; S3, performing preprocessing and multivariate statistical analysis on the original data in sequence; and S4, screening differential metabolites by taking the variable importance projection index as a screening index. According to the method, the differential metabolites of the pseudo-ginseng of different specifications are screened on the basis of integral analysis of the pseudo-ginseng, the UHPLC LTQ Oribitrap MS technology is combined with non-targeted metabonomics and principal component analysis, the method can be used for accurately distinguishing the pseudo-ginseng of different commodity specifications, and a theoretical basis is provided for specification standards of medicinal material commodities in the pseudo-ginseng market.

Description

technical field [0001] The invention relates to the field of analysis and detection of traditional Chinese medicinal materials, in particular to a method for identifying notoginseng with 25 heads and less than 80 heads based on non-targeted metabolomics. Background technique [0002] Panax notoginseng (Burk.) F.H.Chen is the dry root and rhizome of Panax notoginseng (Burk.) F.H.Chen, also known as Shanqi and Jinbuhuan. Radix Notoginseng is sweet in nature and flavor, slightly bitter, warm, returns liver, stomach meridian, function and indications are dispelling blood stasis to stop bleeding, reducing swelling and relieving pain. For coughing up blood, vomiting blood, epistaxis, blood in the stool, metrorrhagia, traumatic bleeding, chest and abdomen tingling pain, tumbling pain, etc. [0003] According to the ministry-promulgated standard "Seventy-six Kinds of Medicinal Materials Product Specification Standards", the specifications and grades of Panax notoginseng can be clas...

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Problems solved by technology

[0004] At present, there are few studies on the internal components of Panax notoginseng with different sizes (weights), mainly including the determination of its polysaccharide content by phenol-sulfuric acid method, and the determination of volatile components by GC-MS. Seven internal components are used as internal indicators to distinguish commercial medicinal materials. Therefore, it is of practical significance to explore the differential metabolites of different sizes and specifications of Panax notoginseng and to find the internal component indicators of Panax notoginseng

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