A kind of medium and culture method for improving tilia callus induction rate

A technology of callus induction and induction medium, which is applied in the field of plant propagation, can solve the problems of long cycle, low breeding efficiency, and difficult cutting breeding, and achieve the effect of improving speed and quality and ensuring breeding efficiency

Active Publication Date: 2022-07-05
JILIN CITY ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the germination rate of linden seed propagation is low, the cycle is long, the cutting propagation is difficult to root, and the survival rate is low. Therefore, it is necessary to study a method of breeding linden with a short cycle and high survival rate.
[0005] Some researchers use Tilia buds as explants to directly induce bud differentiation without callus, which can shorten the culture period. However, using this method to breed Tilia will affect the subsequent rooting speed, rooting rate, transplanting survival rate, and breeding efficiency. lower

Method used

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  • A kind of medium and culture method for improving tilia callus induction rate
  • A kind of medium and culture method for improving tilia callus induction rate
  • A kind of medium and culture method for improving tilia callus induction rate

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Experimental program
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Effect test

Embodiment 1

[0045] A medium for improving the induction rate of tilia callus, comprising:

[0046] (1) Induction medium 1:

[0047] On the basis of MS medium, add 2,4-D 1.5mg / L, zeatin 1.0mg / L, KT 0.15mg / L, proline 20mg / L, leucine 0.8mg / L, chitosan 0.4 g / L;

[0048] and

[0049] (2) Induction medium 2:

[0050] On the basis of MS medium, add 2,4-D 2.5mg / L, zeatin 1.5mg / L, KT 0.2mg / L, sodium thiosulfate 0.15g / L, proline 35mg / L, tyrosine 25mg / L, chitosan 1.0g / L, activated carbon powder (200-300 mesh) 0.5g / L.

[0051] Among them, MS medium is shown in Table 1.

[0052] Table 1

[0053]

[0054]

Embodiment 2

[0056] A medium for improving the induction rate of tilia callus, comprising:

[0057] (1) Induction medium 1:

[0058] On the basis of MS medium, add 2,4-D 1mg / L, zeatin 0.5mg / L, KT 0.2mg / L, proline 15mg / L, leucine 0.5mg / L, chitosan 0.2g / L;

[0059] and

[0060] (2) Induction medium 2:

[0061] On the basis of MS medium, add 2,4-D 2mg / L, zeatin 1mg / L, KT 0.2mg / L, sodium thiosulfate 0.1g / L, proline 30mg / L, tyrosine 20mg / L L, chitosan 0.8g / L, activated carbon powder (200-300 mesh) 0.5g / L.

Embodiment 3

[0063] A medium for improving the induction rate of tilia callus, comprising:

[0064] (1) Induction medium 1:

[0065] On the basis of MS medium, add 2,4-D 2mg / L, zeatin 1.2mg / L, KT 0.1mg / L, proline 25mg / L, leucine 1mg / L, chitosan 0.5g / L L;

[0066] and

[0067] (2) Induction medium 2:

[0068] On the basis of MS medium, add 2,4-D 3mg / L, zeatin 1.8mg / L, KT 0.1mg / L, sodium thiosulfate 0.2g / L, proline 40mg / L, tyrosine 30mg / L, chitosan 1.2g / L, activated carbon powder (200-300 mesh) 0.6g / L.

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Abstract

The invention discloses a medium for improving the induction rate of tilia callus, comprising: (1) induction medium 1: adding 2, 4-D, zeatin, KT, proline on the basis of MS medium , leucine, chitosan; and (2) induction medium 2: on the basis of MS medium supplemented with 2,4‑D, zeatin, KT, sodium thiosulfate, proline, tyrosine, Chitosan, activated carbon powder; and a culture method is disclosed. The culture medium and method of the invention can improve the speed and quality of callus formation, thereby improving the breeding efficiency.

Description

technical field [0001] The invention relates to the technical field of plant reproduction, and more particularly to a medium and a culture method for improving the induction rate of tilia callus. Background technique [0002] Tilia amurensis (Tilia amurensis) belongs to Tiliaceae (Tilia L.) and is a precious protected tree species of Class II in my country. It is mainly produced in northeastern China, eastern Inner Mongolia and the Heilongjiang and Ussuri river basins, south to the Korean Peninsula. . [0003] The material of purple basswood is light and soft, and the texture is dense and straight. It is an important material for excellent plywood and blockboard in the market. Its ecological value is also high. Because of its beautiful tree shape and fragrant flowers, it is a better city and courtyard. Green tree species. At the same time, linden is also a famous nectar source plant, which is a special high-quality nectar source in my country. Tilia flowers and roots can a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40
Inventor 林士杰王梓默朱红波韩姣勾天兵罗也王丽丽张大伟程岩何怀江包广道张忠辉
Owner JILIN CITY ACADEMY OF FORESTRY
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