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Gas response type resonance optical molecularly imprinted sensor and preparation method thereof

A molecularly imprinted, gas-responsive technology, applied in the field of analytical chemical detection

Active Publication Date: 2021-07-06
FOSHAN GREEN INTELLIGENT MFG RES INST OF XIANGTAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But currently, gas-responsive polymers are mostly used for western blotting, never for viral molecular blotting

Method used

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  • Gas response type resonance optical molecularly imprinted sensor and preparation method thereof
  • Gas response type resonance optical molecularly imprinted sensor and preparation method thereof
  • Gas response type resonance optical molecularly imprinted sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1: Preparation of gas-responsive resonant optical molecular imprinting sensor based on zeolite imidazolate material

[0105] Such as figure 1 shown, including the following steps:

[0106] (1) Preparation of zeolite imidazolate material ZIF-8:

[0107] 1.485 g Zn(NO 3 ) 2 ·6H 2 O and 3.28 g of 2-methylimidazole were respectively dissolved in 50 mL of methanol, then the latter solution was quickly poured into the former solution, stirred at room temperature for 12 h, the obtained product was centrifuged, and washed three times with methanol to obtain the product Place in a vacuum oven at 60°C to dry overnight.

[0108] (2) ZIF-8@SiO2 2 Particle preparation:

[0109] Put 0.2 g of ZIF-8 particles in a 250 mL round bottom flask, add 140 mL of absolute ethanol and 20 mL of deionized water. Sonicate for 15 min to mix evenly. Under mechanical stirring, add 8 mL of NH 3 ·H 2 O, then slowly drop 3 mLTEOS, react at room temperature for 12 h, collect by centrifu...

Embodiment 2

[0118] Example 2: Feasibility verification of virus detection by MIP resonant optical sensor

[0119] In order to verify the practicability of the present invention, this embodiment verifies the construction principle of the gas-responsive resonant optical molecular imprinting sensor.

[0120] Such as figure 2 As shown, the resonance light intensity at 470 nm is compared as:

[0121] HBV+ MIPs> MIPs; HBV+ NIPs> NIPs;

[0122] Among them, HBV+ MIPs is the product imprinted polymer particle (MIP) obtained in step (4) of Example 1 to adsorb hepatitis B virus; MIPs is the product imprinted polymer particle (MIP) in step (4) of Example 1; HBV+ NIPs is the embodiment 1. The non-imprinted polymer (NIP) obtained in step (4) adsorbs hepatitis B virus; NIPs is the non-imprinted polymer (NIP) obtained in step (4) of Example 1. The dosage and adsorption conditions of HBV+ MIPs and HBV+ NIPs were the same.

[0123] from Figure 4 It can be seen from the results that the imprinted pol...

Embodiment 3

[0124] Example 3: Characterization of properties, morphology and structure of imprinted polymer particles (MIP) and intermediate products

[0125] To verify the CO of imprinted polymer particles (MIPs) 2 Response performance, in this embodiment, dimethylaminoethyl methacrylate CO was studied by proton nuclear magnetic spectrum 2 Response behavior such as Figure 5 As shown, the picture A is the H NMR spectrum of dimethylaminoethyl methacrylate, and the picture B is the CO 2 The hydrogen nuclear magnetic spectrum of the dimethylaminoethyl methacrylate after that, the picture C shows the N 2 H NMR spectrum of dimethylaminoethyl methacrylate (DMAEMA). Observation reveals that CO 2 Afterwards, the protons of the methyl (d) and methylene functional groups (c) were transferred to the lower field at 2.03 and 2.46–2.49 ppm, and the N 2 Returning to the original position, these observations show that the tertiary amine group of DMAEMA can interact with the CO 2 CO 2 The resultin...

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Abstract

The invention discloses a gas response type resonance optical molecularly imprinting sensor and a preparation method thereof. The gas response type resonance optical molecularly imprinted sensor comprises a carrier and an imprinted polymer, and the imprinted polymer is connected to the carrier; and the imprinted polymer is formed by polymerizing a CO2 response functional monomer and a comonomer, and the imprinted polymer is connected with a target virus template. The carbon dioxide response type imprinted polymer not only improves the specific recognition capability of the sensor on a target virus template, but also effectively avoids the situation that response cannot be achieved due to substance accumulation generated by stimulation compared with imprinted polymers in other chemical stimulation modes.

Description

technical field [0001] The invention relates to the field of analytical chemical detection, and mainly relates to a gas-responsive resonance optical molecular imprinting sensor and a preparation method thereof. Background technique [0002] Recently, the outbreak of novel coronavirus pneumonia (COVID-19) has drawn increasing attention to viral assays. Due to the complex surface conformation and large size of viruses [Alkordi, H., EL-Khamisy S.F., Biosens. Bioelectron., 2017, 92:349-356.], the design and synthesis of its recognition receptors has been a daunting task challenge. Molecularly imprinted polymers are ideal "mimetic antibodies". With the introduction of surface molecular imprinting technology, molecular imprinting technology has developed rapidly in the field of virus detection in recent years. Nevertheless, there are still some deficiencies in the current application of this technology in virus detection, which need to be resolved urgently. Currently, stimuli-r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00B01J20/22B01J20/26B01J20/28B01J20/30
CPCC08G83/001B01J20/06B01J20/226B01J20/268B01J20/103B01J20/28009
Inventor 蔡昌群王凌云杨军玉龚行
Owner FOSHAN GREEN INTELLIGENT MFG RES INST OF XIANGTAN UNIV
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