Identification method and application of M1 type macrophages

A macrophage and identification method technology, applied in the field of cell detection and typing, can solve the problems of insufficient index specificity, time-consuming, difficult clinical transformation, etc., and achieve the effect of high sensitivity and specificity

Active Publication Date: 2021-06-29
XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many detection indicators, insufficient specificity of indicators, and time-consuming shortcomings, and it is not easy to transform into clinical practice

Method used

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  • Identification method and application of M1 type macrophages
  • Identification method and application of M1 type macrophages
  • Identification method and application of M1 type macrophages

Examples

Experimental program
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Effect test

Embodiment 1

[0037] This example is used to illustrate the typing and identification of macrophages, especially M1 type macrophages, by the identification method of the present invention.

[0038] 1. Induction, culture and collection of M0, M1 and M2 macrophages

[0039] Day0: (1) Take two six-well plates and prepare to culture 6-well cells, with two wells each for M0, M1, and M2. Add 1mL of cell culture solution to each well; (2) Take the cells in the culture flask that have been cultured for three days and prepare to subculture, and the cell concentration is about 10 6 cells / mL, add 1 mL of cell suspension to each well; (3) take pictures in a biological safety cabinet (20x, phase contrast mode); (4) add 2 μL of 100 μg / mL PMA to each well (final concentration 100 ng / mL); Induction for 2 days.

[0040] Day2: (1) Take pictures in the biological safety cabinet (20x, phase contrast mode); (2) Replace each well with 2 mL of culture medium; (3) M1 2 wells + 2 μL 100 μg / mL LPS (final concentra...

Embodiment 2

[0050] This example is used to illustrate the typing and identification of macrophages, especially M1 type macrophages, by the identification method of the present invention.

[0051] 1. Induction of M0, M1, and M2 macrophage cells, and intervention with inhibitors of M1 macrophages and agonists of M1 macrophages, cultured and collected.

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Abstract

The invention relates to the technical field of cell detection and typing, and particularly discloses an identification method of M1 type macrophages. According to the method, a micro-fluidic technology is adopted to detect the electrical characteristics of the macrophages from the single cell level, whether a sample to be detected is the M1 type macrophages or not is judged according to the cell membrane specific capacitance of a detection result, or the content or proportion of the M1 type macrophages in the sample to be detected is calculated according to the detection result, and the method can be applied to carry out the high-throughput drug screening of the targeted M1 type macrophages. The method creatively utilizes the difference of cell electrical properties (such as cell membrane unit area capacitance and cytoplasm conductivity) caused by the difference of cells at the aspects of surface topography, cell membrane structure, membrane protein expression, cytoplasm components and the like, achieves the purpose of cell identification and typing, and has higher sensitivity and specificity.

Description

technical field [0001] The invention relates to the technical field of cell detection and typing, in particular to the detection and typing of macrophages. Background technique [0002] Macrophages are mainly derived from bone marrow precursor cells, play an extremely important role in the body's innate immune response and adaptive immune response, and are closely related to inflammatory responses, immune defense, angiogenesis, tumor formation, and internal environment stability. [0003] In different microenvironments, macrophages can differentiate into different cell subpopulations: M1 type and M2 type, the former promotes inflammation and inhibits cell proliferation, and the latter promotes proliferation and tissue repair. Macrophage phenotype switching is closely related to the occurrence, development, and outcome of many diseases, including atherosclerosis, myocardial infarction, cerebral infarction, tumors, asthma, and infectious diseases. For example, decreased M2b m...

Claims

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Application Information

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IPC IPC(8): G01N15/10G01N33/487
CPCG01N15/1031G01N33/48707G01N33/48728
Inventor 赵海苹赵阳黄成军叶一菲栾晓凤李宇昂
Owner XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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