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Method for feeding solution to biological material by using hydrogel

A biomaterial and hydrogel technology, applied in the preservation, application, animal husbandry, etc. of human or animal body, can solve the problem of accidental flushing or sucking of eggs/embryos, avoid the risk of flushing or sucking out, and improve the Reliability and convenience, guarantee the effect of normal operation

Active Publication Date: 2021-06-18
SHEN ZHEN BIOROCKS BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the density of vitrification liquid is higher than that of water, eggs / embryos are usually in a suspended state when immersed in vitrification liquid and drift with the flow of liquid, so that different solutions are put in and sucked out by the robotic arm. risk of accidental flushing or aspiration of eggs / embryos

Method used

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  • Method for feeding solution to biological material by using hydrogel
  • Method for feeding solution to biological material by using hydrogel
  • Method for feeding solution to biological material by using hydrogel

Examples

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Embodiment 1

[0035] In this embodiment, the position of the embryo is fixed, and different solutions are sequentially injected into the area where the embryo is located to complete the contact reaction of the embryo with the basic culture solution, the equilibrium solution and the vitrification solution in sequence.

[0036] combine figure 1 As shown, in the process of embryo freezing using the method of this embodiment, the specific steps of contacting and reacting the embryos with the basic culture medium, the equilibrium solution and the vitrification solution in turn are as follows:

[0037] In step S11, the balance solution and the vitrification solution are respectively prepared into a hydrogel structure.

[0038] In this embodiment, the embryos are pre-placed in the basal culture solution, and then the balance solution and the vitrification solution are put into the basal culture solution in sequence, and the embryos are sequentially mixed with the basal culture solution, basal cult...

Embodiment 2

[0060] In this embodiment, the method of moving the embryos is used, and they are placed on the hydrogel with different solutions in sequence, so as to complete the contact reaction of the embryos with the basic culture solution, the equilibrium solution and the vitrification solution in sequence.

[0061] combine Image 6 As shown, in the process of embryo freezing using the method of this embodiment, the specific steps of contacting and reacting the embryos with the basic culture medium, the equilibrium solution and the vitrification solution in turn are as follows:

[0062] In step S21, the balance solution and the vitrification solution are respectively prepared into a hydrogel structure.

[0063] In this example, in the same manner as in Example 1, the embryos were pre-placed in the basal culture medium, and then contacted with the balance solution and vitrification solution respectively, so that the balance solution hydrogel and glass Thaw the frozen liquid hydrogel. A...

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Abstract

The invention belongs to the technical field of chemical delivery. In order to solve the problems that operation is inconvenient and an ovum / embryo is flushed out of a carrier or directly sucked out when an ovum / embryo and a solution are subjected to contact treatment by adopting a suction tube, the invention discloses a method for feeding a solution to a biological material by utilizing hydrogel. The method specifically comprises the following steps: S1, preparing a solution to be put into a hydrogel structural form; S2, contacting a biological material with the hydrogel prepared in the step S1, and completing the feeding operation through diffusion of a solution in the hydrogel to the biological material. When the method provided by the invention is adopted to carry out the solution feeding operation in the ovum / embryo freezing treatment process, not only can the risk of flushing out or sucking out the embryo possibly caused when a suction tube is used for feeding and sucking out the solution be completely avoided, but also the reliability and the convenience of the feeding operation can be improved, and the normal proceeding of subsequent freezing treatment is ensured.

Description

technical field [0001] The invention belongs to the technical field of chemical delivery, and in particular relates to a method for delivering solution to biological materials using hydrogel. Background technique [0002] By sequentially delivering different solutions into the biological material, the contact reaction between the biological material and different solutions in a specific order is very important in many applications and researches. Among them, during the freezing process of eggs / embryos, it is necessary to first mix the eggs / embryos with basic culture solution (Basic solution, BS), equilibrium solution (Equilibrium solution, ES) and vitrification solution (vitrification solution, VS). The contact reaction is performed before the eggs / embryos are cryopreserved in liquid nitrogen. [0003] At present, when the traditional technology is used to contact the eggs / embryos with different chemicals, the eggs / embryos are usually put into the chemical solution and suck...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 陈昊楠舒怡玮
Owner SHEN ZHEN BIOROCKS BIOTECHNOLOGY CO LTD
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