A colloidal gold test strip for detecting human parathyroid hormone

A parathyroid hormone and test strip technology, applied in the field of biomedical detection, can solve problems such as troublesome operation and long time consumption, and achieve the effects of ensuring activity, reducing morbidity, and being convenient and fast to use

Active Publication Date: 2021-09-21
德林医疗科技(武汉)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of venous blood and the processing of serum require the participation of multiple departments, and the operation is cumbersome and time-consuming

Method used

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  • A colloidal gold test strip for detecting human parathyroid hormone
  • A colloidal gold test strip for detecting human parathyroid hormone
  • A colloidal gold test strip for detecting human parathyroid hormone

Examples

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Embodiment Construction

[0026] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0027] 1. Antibody screening and preparation

[0028] The whole protein of parathyroid hormone was used to immunize alpacas to prepare anti-human iPTH antibody. Mix and emulsify 250 μg antigen with 250 μL Freund’s complete adjuvant, and use the mixture to immunize alpacas to complete the primary immunization. After 2 weeks and 4 weeks after the primary immunization, the alpacas were given a booster immunization, and the mixture of 1250 μg antigen and 250 μL Freund's incomplete adjuvant was used for booster immunization. One week after the second and third immunizations, blood was collected to determine the titer of antiserum.

[0029] The results showed that the antiserum titers elicited by the second and third immunizations of the antigen were 3.28×10 6 and...

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Abstract

The invention relates to a test paper strip for detecting human parathyroid hormone, comprising a base plate, and a sample pad, a colloidal gold binding pad, a nitrocellulose membrane and a water-absorbing pad arranged on the base plate and sequentially connected, the nitrocellulose A detection line is set on the membrane; the colloidal gold binding pad contains colloidal gold particles labeled with the binding antibody; the detection line is anchored with a capture antibody, and both the binding antibody and the capture antibody can specifically recognize and bind to parathyroid hormone, and bind to The binding site of the antibody and the capture antibody to parathyroid hormone does not interfere with each other. The colloidal gold test strip of the present invention is convenient and quick to use, greatly shortens the detection time, thereby helping doctors judge whether to cut parathyroid glands by mistake in a short period of time, greatly ensuring the activity of parathyroid glands, and greatly reducing the risk of thyroid glands after thyroid surgery. Incidence of paragonal hypofunction. In addition, the colloidal gold of the present invention can also be used to detect whole blood samples and can be used for the diagnosis of parathyroid cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a colloidal gold test strip for detecting human parathyroid hormone. Background technique [0002] There are two pairs of parathyroid glands in the human body, which are brown and yellow in shape, and are located in the middle and lower parts of the back (or buried in) of the left and right thyroid glands. The main function is to secrete parathyroid hormone (referred to as PTH), and regulate the metabolism of calcium and phosphorus in the body. Diseases related to parathyroid glands are mainly hypoparathyroidism after thyroid surgery and primary or secondary parathyroid adenoma. [0003] Thyroid cancer is a common malignant tumor that occurs in the head and neck, and it is also one of the fastest-growing malignant tumors in recent years. Thyroid cancer has become the fifth most common malignant tumor in women. The treatment of thyroid cancer is currentl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/58G01N33/558
CPCG01N33/558G01N33/587G01N33/74G01N2333/635
Inventor 李兴睿
Owner 德林医疗科技(武汉)有限公司
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