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Zm0001d024568 gene and application of encoding protein thereof in corn drought stress resistance

A gene encoding and anti-drought technology, which is applied in the fields of application, genetic engineering, and the introduction of foreign genetic material using vectors, etc., can solve the problems of relatively few studies on important functions and regulatory components of drought stress, and no gene research reports have yet been seen.

Active Publication Date: 2021-05-14
HENAN UNIVERSITY
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Problems solved by technology

In long-term drought research, many components that play an important role in plant drought stress responses have been identified, but the identification of these components and the research on their functional pathways are mainly realized through the model Arabidopsis, etc. There are relatively few studies on the important functions and regulatory components of drought stress in the important crops maize, rice, and wheat. It is of more theoretical and practical significance to identify the important components and drought response pathways in their response to drought stress
[0003] The protein encoded by the maize Zm0001d024568 gene is a member of the MAPK protein kinase family. The CDS of the Zm0001d024568 gene is 1044bp in length and encodes a 348-amino acid MAPK protein kinase. However, there is no research report on the gene's role in stomatal movement and drought stress response

Method used

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  • Zm0001d024568 gene and application of encoding protein thereof in corn drought stress resistance
  • Zm0001d024568 gene and application of encoding protein thereof in corn drought stress resistance
  • Zm0001d024568 gene and application of encoding protein thereof in corn drought stress resistance

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Embodiment 1

[0030] The CDS of maize Zm0001d024568 gene is 1044bp in length, encodes a protein of 348 amino acids, and belongs to the MAPK protein kinase family.

[0031] The maize-related mutants come from a maize mutant library constructed in the laboratory, and the mutant of the maize Zm0001d024568 gene is confirmed to be a coding sequence early termination mutant (dos59) through sequencing.

[0032] 1. Obtaining a homozygous dos59 mutant with premature termination of the Zm0001d024568 gene coding sequence

[0033] Firstly, obtain the Zm0001d024568 gene coding sequence premature termination homozygous dos59 mutant from the corn mutant library, cultivate the seedlings of the Zm0001d024568 mutant strain in a greenhouse, the light / dark cycle is 14 / 10h, and the temperature day / night: 30 / 24°C , germinate and grow under the condition of relative humidity of 70%. Utilize SLS (sodium lauryl sarcosine) method to extract leaf DNA, take it as template, specific primer comprises forward primer and...

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Abstract

The invention relates to the technical field of gene engineering application, particularly discloses application of Zm0001d024568 gene encoding protein in corn drought stress resistance, and finds that the corn Zm0001 d024568 gene can be used for regulating and controlling corn leaf surface temperature, regulating and controlling corn stomatal movement, influencing corn stomatal opening and stomatal conductance and regulating and controlling plant drought tolerance. The Zm0001d024568 gene disclosed by the invention can be used for functional identification in corn plant stomatal movement regulation and plant drought stress response, so that a theoretical foundation is laid in the aspect of creating corn germplasm resources with strong drought tolerance.

Description

technical field [0001] The invention relates to the technical field of genetic engineering application, in particular to the application of the Zm0001d024568 gene and its encoded protein in corn drought stress resistance. Background technique [0002] Drought is the most important limiting factor of crop yield and has become one of the central topics of plant adversity stress. Corn has become the world's largest crop, and its yield and quality affect the survival and development of human society. In long-term drought research, many components that play an important role in plant drought stress responses have been identified, but the identification of these components and the research on their functional pathways are mainly realized through the model Arabidopsis, etc. There are relatively few studies on the important functions and regulatory components of drought stress in the important crops maize, rice, and wheat. It is of more theoretical and practical significance to ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/82A01H5/12A01H5/00A01H6/46
CPCC12N9/1205C12N15/8273
Inventor 李保珠宋纯鹏刘炯张辉陈婷婷刘茹南
Owner HENAN UNIVERSITY
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