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Experimental method for rabbit KOA model chondrocytes by adopting medicine scraping method

A chondrocyte and experimental method technology, applied in the research field of knee cartilage cells, can solve problems such as knee joint degenerative disease and cell apoptosis

Pending Publication Date: 2021-04-16
湖南省中医药研究院
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Problems solved by technology

Among them, in the process of apoptosis mediated by mitochondria / cytochrome C, once the cells are damaged, the mitochondria will spontaneously release cytochrome C, and the precursors of Apaf-1 and caspase-9 will combine with it, so that caspase- 9 activation, and then activate caspase-3, and start the caspase cascade reaction, thereby inspiring the chain reaction of various signaling factors in the entire apoptotic pathway, leading to cell apoptosis and eventually knee joint degeneration occurrence of lesions

Method used

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  • Experimental method for rabbit KOA model chondrocytes by adopting medicine scraping method
  • Experimental method for rabbit KOA model chondrocytes by adopting medicine scraping method
  • Experimental method for rabbit KOA model chondrocytes by adopting medicine scraping method

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Embodiment Construction

[0044] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0045] Select 40 experimental white rabbits, and feed them normally for 7 days before the experiment. After observing that there is no abnormality, they are randomly numbered (No. 1-40), and then randomly divided into 5 groups in strict accordance with the SPSS22.0 statistical software: (A) normal Group, (B) model control group, (C) treatment group: iron-coated gold massage cream + scraping group, (D) control group: iron-coated gold massage cream group, (E) control group: scraping group. 8 in each group. The normal group only received conventional feeding without modeling or intervention, and the other four groups received intra-articular injection of papain to make a rabbit knee osteoarthritis model. The intervention was performed 2 weeks after the model was made, and the materials were obtained 20 days after the intervention. The ...

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Abstract

The invention provides an experimental method for rabbit KOA model chondrocytes by adopting a medicine scraping method, which is characterized by comprising the following steps: 1, establishing a rabbit KOA model; 2, regularly smearing the knee joint of the rabbit with the berchemia lineate massage cream by adopting a medicine scraping method; 3, preparing an animal specimen; 4, performing morphological observation on cartilage tissue in the specimen; and 5, carrying out immunohistochemical detection on caspase-3, caspase-9 and XIAP proteins in the cartilage tissue. The research of the invention proves that the medicine scraping method can reduce the expression quantity of caspase-3 and caspase-9 in the rabbit articular cartilage of the K0A model and up-regulate the content of XIAP in cytobiology, so that the articular cartilage is protected from being damaged, and the joint senescence is delayed.

Description

technical field [0001] The invention relates to a research method for knee joint chondrocytes, in particular to an experimental method for treating rabbit KOA model chondrocytes by drug scraping. Background technique [0002] Osteoarthritis (OA) is a chronic, progressive, and degenerative clinical common and frequently-occurring disease. With the aging of the population, the incidence of OA is increasing year by year. According to domestic and foreign epidemiological literature, OA The prevalence rate is about 15%, and it is as high as 50% for those older than 60 years old, and the final disability rate reaches 53%. The World Health Organization (WHO) lists OA, cardiovascular disease, and cancer as the three major killers that endanger human health, which have brought heavy burdens to society and families. Among OA, knee osteoarthritis (KOA) is the most common disease. Because the pathogenesis of knee osteoarthritis is not clear, many clinical treatment methods cannot fund...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/68G01N1/28G01N1/36G01N21/84
Inventor 匡建军蔡萍戎宽匡浩铭杨惠胡余飞曹闲雅谭学松沈琳玲
Owner 湖南省中医药研究院
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