A kind of culture medium for cultivating Aconitum grass

A tissue culture and culture medium technology, applied in the directions of tissue culture, application, microorganisms, etc., can solve the problems of reducing the browning rate of callus and the survival rate of seedlings, long period of seedling raising in sand, shortening the time of raising seedlings, etc., and achieves good application. Prospects, the effect of improving the survival rate of seedlings and reducing the induction rate

Active Publication Date: 2022-04-08
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to make up for the deficiencies in the prior art, the present invention provides a culture medium for Aconitum radix tissue, which realizes the functions of effectively reducing the browning rate of callus and high seedling survival rate, and solves the problem of traditional sand storage treatment. The problems of low survival rate of seedlings and long cycle of raising seedlings in sand can improve the survival rate of seedlings and shorten the time of seedlings; the seedling pollution rate is reduced to below 3%, the browning rate is reduced to 0%, and the number of rooted bars reaches 45% by adopting the technology of the present invention. -60 sticks / bottle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The primary medium is: GS+6-BA 1mg / L+NAA 0.45mg / L+AC 5mg / L+KT 0.05mg / L;

[0036] The subculture medium is: improved MS+6-BA 3mg / L+NAA 0.45mg / L+AC 5mg / L, the formula of the improved MS medium is potassium nitrate and ammonium nitrate changed to 1 / 3 of the original; organic components The VB1 in the product is increased by 1 / 2 on the original basis; the manganese sulfate in the trace elements is reduced by 1 / 4, and the copper sulfate is removed;

[0037] The rooting medium formula is 1 / 2MS+6-BA 0.095mg / L+B9 0.5mg / L;

[0038] The cultivation process is as follows:

[0039] S1: Select Aconitum aconitifolia branches with soft and tender skin as explants, and the size of the explants is 3-5mm;

[0040] S2: Disinfect the selected explants. The specific method is: first soak with agricultural streptomycin for 5 minutes, then rinse with sterile water for 5 times, each time for 60 seconds, then soak in 0.1% mercuric solution for 8 minutes, and then use Rinse the sterile water ...

Embodiment 2

[0048] The primary medium is: GS+6-BA 1mg / L+NAA 0.5mg / L+AC 5mg / L+KT 0.05mg / L;

[0049] The subculture medium is: improved MS+6-BA 3mg / L+NAA 0.5mg / L+AC 5mg / L, the formula of the improved MS medium is potassium nitrate and ammonium nitrate changed to 1 / 3 of the original; organic components VB in 1 Increase by 1 / 2 on the original basis; reduce manganese sulfate in trace elements by 1 / 4, and remove copper sulfate;

[0050] The rooting base formula is 1 / 2MS+6-BA 0.1mg / L+B9 0.5mg / L;

[0051] All the other steps are the same as in Example 1.

[0052] Example 2

[0053] The primary medium is: GS+6-BA 1mg / L+NAA 0.55mg / L+AC 5mg / L+KT 0.05mg / L;

[0054] The subculture medium is: improved MS+6-BA 3mg / L+NAA 0.55mg / L+AC 5mg / L, the formula of the improved MS medium is potassium nitrate and ammonium nitrate changed to 1 / 3 of the original; organic components VB in 1 Increase by 1 / 2 on the original basis; reduce manganese sulfate in trace elements by 1 / 4, and remove copper sulfate;

[0055]...

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PUM

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Abstract

The invention discloses a culture medium for cultivating Aconiti radix tissue, comprising primary culture medium, secondary culture medium and rooting medium. The primary culture medium is GS+6-BA 1mg / L+NAA 0.45-0.55mg / L+AC 5mg / L+KT 0.05mg / L; the subculture medium is improved MS+6-BA 3mg / L+NAA 0.45-0.55mg / L+AC 5mg / L; the rooting medium formula 1 / 2MS+6‑BA 0.095‑0.105mg / L+B 9 0.5mg / L. Through the cultivation method of the invention, the functions of effectively reducing the browning rate of callus and high survival rate of seedlings are realized, the problem of low survival rate of seedlings raised by traditional sand storage is solved, and the survival rate of seedlings is improved.

Description

technical field [0001] The invention relates to the field of biology, in particular to a culture medium for cultivating Aconitum grass. Background technique [0002] Aconitum, the name of traditional Chinese medicine; it is the dry tuberous root of Aconitum aconitum of the Ranunculaceae plant; excavated when the stems and leaves wither in autumn, remove fibrous roots and sediment, and dry; cultivate means to cultivate young organisms to make them grow; used for wind-cold-damp arthralgia , joint pain, confidant cold pain, cold colic pain and anesthesia pain relief; used to treat cold-damp blood stagnation in the meridian, limb muscle spasm pain, joint flexion and extension: same use with Chuanwu, earthworm, frankincense, etc.; often used as anesthesia Painkillers are mostly used together with raw products and raw Aconitum, and are compatible with sheep's weed and turmeric. [0003] Before germination of Aconitum aconitum seeds, sand storage treatment is required. After sand ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 马怀林贾振宇张跃华高俊刚刘亚玲屈璐璐刘思泱高旭王乌恩巴图
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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