Snps loci and applications related to appetite suppression in largemouth bass

A technology of largemouth bass and molecular markers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., and can solve the problems of further verification of biological functions

Active Publication Date: 2022-08-02
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

However, the biological functions of these candidate genes and genotypes have not been further validated in largemouth bass cultured on alternative feeds

Method used

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  • Snps loci and applications related to appetite suppression in largemouth bass
  • Snps loci and applications related to appetite suppression in largemouth bass
  • Snps loci and applications related to appetite suppression in largemouth bass

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Embodiment Construction

[0057] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art fall within the protection scope of the present invention.

[0058] Unless otherwise defined, all technical terms used hereinafter have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are only for the purpose of describing specific embodiments, and are not intended to limit the protection scope of the present invention.

[0059] Unless otherwise specified, the various reagents and raw materials used in the present invention are commercial products that can be purchased from the market or products that can be prepared by known methods.

[0060] Materials and...

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Abstract

The invention provides a SNPs site and application in POMC of largemouth bass, belonging to the fields of aquatic breeding and biological gene technology. The present invention explores the endocrine physiology associated with POMC gene polymorphisms in largemouth bass under fasting and refeeding conditions. The single-gene sequence of POMC was obtained from a transcriptome dataset annotated with SNP sites, the full-length POMC cDNA was cloned, and the structure and homology of the gene were analyzed. The loci and genotypes of SNPs in largemouth bass POMC were determined, and the frequency distribution of different genotypes in populations raised on different diets was calculated. Expression patterns of POMC genotypes were compared after fasting and refeeding. Domestication of largemouth bass by replacing forage fish with formula feed has been well developed in the aquaculture industry, and this study will benefit molecular marker-assisted breeding of largemouth bass feeding habits for fast-growing, high-efficiency formula feeds. Utilization and high domestication efficiency feeding traits in largemouth bass populations.

Description

technical field [0001] The invention relates to the technical fields of aquatic breeding and biological genes, and relates to a SNPs site related to appetite suppression in largemouth bass and its application. Background technique [0002] Largemouth bass (Micropterus salmoides) is now one of the most productive species in aquaculture, with an annual production of 477,808 tonnes in 2019 (China Fisheries Statistical Yearbook 2020). Wild largemouth bass are carnivorous fish that prefer to prey on small fish, crayfish, amphibians and insects in their natural environment, while commercially farmed largemouth bass feed on frozen or live forage fish. Forage fish cause huge production costs and over-exploitation of marine resources, and at the same time, excessive forage fish can drastically deteriorate water quality. To mitigate the negative effects of fishmeal, there is inevitably a trend towards the use of formula feeds as a substitute in the production of largemouth bass. Lar...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2527/107
Inventor 胡婕孙承熙叶星
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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