Preparation method of anisin, detection method of anisin and antibacterial application of anisin

Abstract

The invention discloses a preparation method of anisin, a detection method of anisin and antibacterial application, the preparation method of anisin comprises the following steps: inoculating a strain seed solution of streptomyces albidoflavus CK-15 into a seed culture medium, culturing at 28 DEG C at 220rpm for 24h, transferring into 100ml of a fermentation medium according to an inoculation amount of 5%, fermenting at 30 DEG C for 5d, centrifuging the obtained fermentation liquid, collecting a first supernatant; adjusting the pH value of the first supernatant to 9.0, and extracting the first organic phase by using ethyl acetate with the same volume as the supernatant; carrying out reverse extraction on the first organic phase by using acid water with the same volume as the first organic phase and the pH value of 2.0, collecting a water phase and adjusting the pH value of the water phase to 9.0; extracting with ethyl acetate with the volume equal to that of water, collecting a second organic phase and spin-drying; according to the preparation method of the anisimycin, the streptomyces albidoflavus CK15 strain is fermented to prepare the anisimycin, and compared with a chemical synthesis method for preparing the anisimycin, the preparation method is simple, convenient to operate and low in cost.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing anisomycin through fermentation, a detection method for anisomycin and an antibacterial application. Background technique [0002] Anisomycin is a nitrogen-containing heterocyclic aromatic antibiotic produced by actinomycetes such as Streptomyces griseus. It has good antifungal, antiprotozoal and antitumor effects. At present, it is widely used in the research of cell and molecular genetics, and has great potential as an immunosuppressive drug. [0003] At present, most anisomycins are prepared by chemical synthesis, which has many problems such as high cost of chiral catalysts, harsh reaction conditions, difficult separation of enantiomers, and serious environmental pollution. Contents of the invention [0004] Aiming at the problems existing in the prior art, the first object of the present invention is to provide a method for preparing ...

AI Technical Summary

Problems solved by technology

[0003] At present, most anisomycins are prepared by chemical synthesis, which has many problems such as high cost of chiral catalysts, harsh reaction conditions, difficult separation of enantiomers, and serious environmental pollution.

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