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Atpep1 mature polypeptide plant aging accelerator and its preparation method and application

A mature polypeptide, plant senescence technology, applied in the fields of botanical equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problem of no plant senescence, and achieve convenient operation, convenient acquisition, and strong field operability. Effect

Active Publication Date: 2021-09-03
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the application of mature polypeptides in plants, especially crops, and there is no research on their application in promoting plant senescence.

Method used

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  • Atpep1 mature polypeptide plant aging accelerator and its preparation method and application
  • Atpep1 mature polypeptide plant aging accelerator and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0029] AtPep1 mature polypeptide plant senescence accelerator, including 10 μmol / L AtPep1 mature polypeptide, 2.8mmol / L 2-(N-morpholine)ethanesulfonic acid solution; the 2-(N-morpholine)ethanesulfonic acid solution is 2- (N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution was 5.8.

[0030] The preparation method is as follows:

[0031] (1) Weigh AtPep1 mature polypeptide powder and dissolve it in water, and prepare AtPep1 mature polypeptide mother solution with a concentration of 10mmol / L for later use;

[0032] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above-mentioned MS liquid medium, prepare a 2.8mmol / L solution, adjust the pH value of the solution to 5.8, mix and dissolve Uniformly obtain 2-(N-morpholine) ethanesulfonic acid solution;

[0033] (3) Take 40 μL of the AtPep1 mature polypeptide mother solution obtained in step (1) and a...

Embodiment 2

[0035] AtPep1 mature polypeptide plant aging promoter, including 12 μmol / L AtPep1 mature polypeptide, 2.9mmol / L 2-(N-morpholine)ethanesulfonic acid solution; the 2-(N-morpholine)ethanesulfonic acid solution is 2- (N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution was 5.9.

[0036] The preparation method is as follows:

[0037] (1) Weigh AtPep1 mature polypeptide powder and dissolve it in water, and prepare AtPep1 mature polypeptide mother solution with a concentration of 10mmol / L for later use;

[0038] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above MS liquid medium, prepare a 2.5mmol / L solution, adjust the pH value of the solution to 5.9, mix and dissolve Uniformly obtain 2-(N-morpholine) ethanesulfonic acid solution;

[0039] (3) Take 120 μL of the AtPep1 mature polypeptide mother liquor obtained in step (1) and add it to 100 mL of th...

Embodiment 3

[0041] AtPep1 mature polypeptide plant senescence accelerator, including 13 μmol / L AtPep1 mature polypeptide, 3mmol / L 2-(N-morpholine) ethanesulfonic acid solution; the 2-(N-morpholine) ethanesulfonic acid solution is 2-( N-morpholine) ethanesulfonic acid solution monohydrate is dissolved in MS liquid medium, and the pH of 2-(N-morpholine) ethanesulfonic acid solution is 5.8.

[0042] The preparation method is as follows:

[0043] (1) Weigh AtPep1 mature polypeptide powder and dissolve it in water, and prepare AtPep1 mature polypeptide mother solution with a concentration of 10mmol / L for later use;

[0044] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above MS liquid medium, prepare a 3mmol / L solution, adjust the pH value of the solution to 5.8, mix and dissolve evenly Obtain 2-(N-morpholine)ethanesulfonic acid solution;

[0045] (3) Take 78 μL of the AtPep1 mature polypeptide mother solution obtained in step (1) and add it to 60 mL o...

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Abstract

The present invention proposes an AtPep1 mature polypeptide plant aging accelerator and its preparation method and application, belonging to the field of plant aging accelerators. The AtPep1 mature polypeptide plant aging accelerator uses AtPep1 mature polypeptide as the main functional component. The AtPep1 mature polypeptide has the following Amino acid sequence: A‑T‑K‑V‑K‑A‑K‑Q‑R‑G‑K‑E‑K‑V‑S‑S‑G‑R‑P‑G‑Q‑H‑N. The plant senescence accelerator provided by the invention can accelerate the senescence of leaves of plants and accelerate the senescence without other additional adverse performances, and has strong operability in the field.

Description

technical field [0001] The invention belongs to the field of plant aging accelerators, and in particular relates to an AtPep1 mature polypeptide plant aging accelerator and its preparation method and application. Background technique [0002] Leaf senescence is an important factor affecting crop yield, quality and other agronomic traits. Taking tobacco as an example, the leaves are the harvesting organs of tobacco. As the tobacco enters the late growth stage, the leaves enter the aging stage, and the maturity stages of the upper, middle, and lower leaves of tobacco are different. Harvest time, and the use of aging accelerators can effectively solve the problems of inconsistent maturity and greening of tobacco leaves in different parts. [0003] At present, the application of plant aging control mainly focuses on the regulation of plant hormones. For example, auxin and cytokinin can inhibit leaf aging, while ethylene and abscisic acid can promote leaf aging. aging process. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/46A01N43/84A01P21/00
CPCA01N37/46A01N43/84Y02A40/146
Inventor 郭永峰王奇郭存李伟张增林文利超邓智超刘涛
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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