Method for producing ethanol by glucose fed-batch fermentation based on online ethanol concentration response value monitoring

Abstract

The invention discloses a method for producing ethanol by glucose fed-batch fermentation based on online ethanol concentration response value monitoring. The method comprises the following steps: a step of conducting seed culture; and a step of producing ethanol by fermentation culture, wherein in the step of producing the ethanol by fermentation culture, the online ethanol concentration responsevalue monitoring is carried, and when the ethanol concentration response value is in a descending trend, glucose feeding is started to be carried out, and fermentation is continued. According to the method disclosed by the invention, the electronic olfactory is introduced into the ethanol fermentation process, and the relationship between the electronic olfactory and the ethanol concentration is established to prove that the electronic olfactory can realize the online monitoring of key indexes and parameters, especially the product ethanol concentration, in the fermentation process, so that glucose is added in stages in the ethanol fermentation process in an online guidance manner, and the inhibition effect on cell growth and ethanol synthesis in the fermentation process is relieved, thuseffectively improving the ethanol production efficiency and the sugar-ethanol conversion rate.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing ethanol by glucose fed-feed fermentation based on online ethanol concentration response value monitoring. Background technique [0002] With the consumption of fossil fuels and the escalation of environmental pollution and global warming caused by the use of fossil fuels, biofuels are considered to be the most potential renewable biomass energy in order to cope with global climate change and energy security. risks of. Replacing fossil fuels with biofuel ethanol can reduce 90% of car carbon dioxide emissions, thus showing better environmental friendliness, but there are still certain disadvantages in terms of economy. Therefore, the development of efficient fuel ethanol fermentation technology is the key to achieve low-cost production. [0003] Process detection plays an important role in the understanding and regulation of fermentation proc...

AI Technical Summary

Problems solved by technology

However, at present, the control of industrial ethanol fermentation process is still based on manual experience, and the online detection parameters of the process are limited to temperature, pH, etc., so it is impossible to realize the online monitoring of key index parameters, especially the monitoring of product ethanol

[0004] At present, the commonly used method for ethanol detection is liquid chromatography, but there are problems such as off-line, high cost, long detection time, and the use of organic solvents.

Although it has also been reported that glucose and ethanol in the ethanol production process can be monitored simultaneously by using near-infrared spectroscopy combined with partial least squares regression, but this method requires the use of expensive near-infrared instruments and the establishment of corresponding models for different fermentation systems , there are certain application limitations

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