The near-infrared fluorescent probe substrate of comt and its application
A fluorescent probe and probe substrate technology, applied in the field of biomedicine, can solve problems affecting the accuracy of enzyme activity, weak anti-interference ability, substrate oxidation degradation, etc., achieve good fluorescence emission characteristics, strong anti-interference ability, The effect of improving accuracy
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[0036] 1. Synthesis of TCFC:
[0037] Mix 3-formyl-7,8-dihydroxycoumarin (0.41g, 2mmol) with 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-alkylene) Malononitrile (TCF) (0.45g, 2.3mmol) was dissolved in methanol (40ml), piperidine (100μl) was added, the reaction leaf was heated to reflux for 2h, filtered after cooling, the filter cake was recrystallized from methanol, and the crude product was subjected to column chromatography ( dichloromethane / methanol) to obtain the product TCFC, 110 mg of red solid, yield 15%. HRMS(TOF-ESI):m / z C 21 h 13 N 3 o5 :387.0855, mass spectrum shows m / z386.0783[M-H] - .
[0038] 2. Characterization of the optical properties of the probe substrate TCFC and its methylated product TCFMC:
[0039] (1) The probe substrate TCFC and its methylated product TCFMC were dissolved in pH 7.4 Tris-HCl buffer (50 mM) and 1% formic acid-acetonitrile (v / v 1:1) mixture.
[0040] (2) Take 200 μL in a transparent 96-well plate, and scan its absorption spectrum from 20...
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