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Chlorella kessleri mutant, culture method therefor and application of chlorella kessleri mutant in livestock and poultry breeding wastewater treatment

A culturing method and chlorella technology, applied in the biological field, can solve the problems of land consumption overload, secondary pollution, etc., and achieve the effect of high degree of harmlessness and high resource gain.

Inactive Publication Date: 2021-03-09
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Claims
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AI Technical Summary

Problems solved by technology

The biogas slurry is directly returned to the field for irrigation or fertilizer use without advanced treatment, which will easily cause land consumption overload and cause secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Algae strain: chlorella mutant strain, the strain preservation registration number is CGMCC No. 4917, and the classification name is Chlorella ( Chlorella kessleri ) A1 CGMCC No.4917, which was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms on May 27, 2011.

[0019] Cultivation method and application: The cultivation system uses an indoor paddle track pool, the cultivation temperature is 25°C, and the continuous light is 100 μmol m -2 the s -1 , pH 7. The initial concentration of ammonia nitrogen in biogas slurry was adjusted to 100 mg L -1 Afterwards, it was used as the initial medium for the growth of Chlorella. The air compressor continuously feeds the ambient air into the algae liquid to supplement the carbon dioxide and oxygen required for the cultivation system. The minimum ammonia nitrogen concentration in the culture medium is reduced to 5 mg L -1 When the time is below, 30% of the culture fluid is coll...

Embodiment 2

[0021] Algae strain: chlorella mutant strain, the strain preservation registration number is CGMCC No. 4917, and the classification name is Chlorella ( Chlorella kessleri ) A1 CGMCC No.4917, which was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms on May 27, 2011.

[0022] Cultivation method and application: Indoor pipeline photobioreactor was selected for the cultivation system, the cultivation temperature was 30°C, and the continuous light was 80 μmol m -2 the s -1 , pH 6.5. The initial concentration of ammonia nitrogen in biogas slurry was adjusted to 50 mg L -1 Afterwards, it was used as the initial medium for the growth of Chlorella. The air compressor continuously feeds the ambient air into the algae liquid to supplement the carbon dioxide and oxygen required for the cultivation system. The minimum ammonia nitrogen concentration in the culture medium is reduced to 1 mg L -1 When the time is below, 15% of the cul...

Embodiment 3

[0024] Algae strain: chlorella mutant strain, the strain preservation registration number is CGMCC No. 4917, and the classification name is Chlorella ( Chlorella kessleri ) A1 CGMCC No.4917, which was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms on May 27, 2011.

[0025] Cultivation method and application: The cultivation system adopts optical fermenter, the cultivation temperature is 26℃, and the continuous light is 120μmol m -2 the s -1 , pH 6.8. The initial concentration of ammonia nitrogen in biogas slurry was adjusted to 100 mg L -1 , add glucose to a final concentration of 10 g L -1 , after autoclaving, as the initial growth medium for Chlorella. The air compressor continuously injects sterile ambient air into the algae liquid to supplement the carbon dioxide and oxygen needed for the cultivation system. Add 50% glucose sterile solution intermittently to the culture medium to maintain the glucose concentration ...

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Abstract

The invention discloses a chlorella kessleri mutant and a culture method therefor and belongs to the technical field of livestock and poultry breeding wastewater treatment. The chlorella kessleri mutant disclosed by the invention has an accession number of CGMCC No. 4917, has a classification name of chlorella kessleri A1 CGMCC No. 4917 and is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on May 27, 2011. The culture method for the chlorella kessleri mutant can be applied to a deep treatment process on digestedeffluent, so as to maximally achieve harmless and resource-converting utilization of manure of livestock and poultry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chlorella mutant strain, a cultivation method thereof and its application in the treatment of livestock and poultry breeding wastewater. Background technique [0002] The livestock and poultry breeding industry accounts for one-third of the total output value of my country's agriculture, forestry, animal husbandry and fishery. It is a large industry that is related to food supply and public health safety, solves the employment of more than 200 million people, increases farmers' income, and drives the development of many related industries. The rapid development of livestock and poultry breeding industry and the continuous improvement of scale and intensification have gradually highlighted the contradiction between livestock and poultry manure and the environment. In order to meet consumers' demand for fresh livestock and poultry products and reduce transportation and sa...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12R1/89
CPCC12N1/20C02F3/322
Inventor 王艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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