Application of protein m57 in regulating rice resistance to ammonium

Abstract

The invention discloses the application of protein M57 in regulating the resistance of rice to ammonium. A recombinant vector expressing protein M57 was introduced into Nipponbare to obtain T 0 Transgenic rice with OsMADS57‑R gene; T 0 Transgenic OsMADS57‑R gene rice was continuously selfed for three generations to obtain T 3 Generation of homozygous transgenic OsMADS57‑R rice. Compared with Nippon Clear, T 3 The generation homozygous OsMADS57‑R transgenic rice had stronger growth in the vegetative growth period, significantly increased leaf width, significantly thicker stems, more compact plant shape, significantly increased leaf angle, and significantly decreased plant height at the mature stage. Grain weight, grain width and yield increased significantly after harvesting, as did panicle length and grain number per panicle. It can be seen that protein M57 plays an important role in regulating rice resistance to ammonium, yield, plant height, grain weight, grain width, panicle length, grain number per panicle, leaf width, stem diameter and leaf angle. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of protein M57 in regulating the resistance of rice to ammonium. Background technique [0002] Rice is one of the most important food crops in the world. More than 120 countries around the world grow rice, and the cultivated area is maintained at more than 150 million hectares all year round. 50% of the world's population uses rice as a staple food. The yield of rice is very dependent on the application of nitrogen fertilizer, but the application of nitrogen fertilizer also has many serious negative effects. For example, most of the applied nitrogen fertilizer cannot be effectively used by rice, but seeps into groundwater and volatilizes into groundwater in the form of various forms of nitrogen compounds. In the air, the nitrogen cycle causes pollution and damage to large areas of water resources, soil and ecosystems. In addition, rice is a crop that prefe...

AI Technical Summary

Problems solved by technology

The yield of rice is very dependent on the application of nitrogen fertilizer, but the application of nitrogen fertilizer also has many serious negative effects. For example, most of the applied nitrogen fertilizer cannot be effectively used by rice, but seeps into groundwater and volatilizes into groundwater in the form of various forms of nitrogen compounds. In the air, the nitrogen cycle causes pollution and destruction of large areas of water resources, soil and ecosystems

In addition, rice is a crop that likes ammonium nitrogen fertilizers, but excessive ammonium fertilizers are toxic to rice, affecting the growth and yield of rice.

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