Culture method of nardostachys jatamansi callus cells

Abstract

The invention belongs to the field of tissue culture, and particularly relates to a culture method of nardostachysjatamansi callus cells. The culture method comprises the following steps: A, washing and disinfecting petioles and leaves of wild nardostachys jatamansi to obtain base tissue; B, cutting the base tissue prepared in the step A, and inoculating a callus induction culture medium with thecut base tissue for culturing to obtain callus; and C, inoculating a callus subculture medium with the callus prepared in the step B for subculture to obtain subculture callus cells. According to theculture method, the leaves and petioles of the nardostachys jatamansi plant are used for inducing the callus cells of the nardostachys jatamansi plant, and the callus cells with high secondary metabolite (chlorogenic acid and polyphenol) content are obtained under low-temperature stimulation induction; and the method can be used for obtaining a large number of nardostachys jatamansi embryogenic callus cells containing active substances in a short time, has the characteristics of safety, no toxicity, short period, high propagation speed, simplicity in operation and low cost, and provides convenience for application of the nardostachys jatamansi extract in actual products.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a culture method of nard pine callus cells. Background technique [0002] Plant cell tissue culture (plant cell and tissue culture) refers to a technique of separating one or several individual cells or a part of a plant body under sterile conditions to undergo dedifferentiation, callus formation, and redifferentiation. Using the plant cell tissue culture method, a large number of secondary metabolites can be extracted from the cultured plant cells or callus under controlled and repeated conditions, and can provide enzyme preparations, pharmaceutical products, and additives for humans. The production of plant secondary metabolites using large-scale plant cell culture technology has been widely used. On the one hand, it can overcome the different growth conditions of the geographical environment, climate, soil, etc., as well as the different time of harvesting medicinal m...

AI Technical Summary

Problems solved by technology

However, due to the international endangered plant of N. pine, the project only uses the rhizome and buds of N. pine as explants. Not only is it easy to damage the N. pine plant, but also the disinfection and sterilization steps are complicated, and there has been no report so far that the whole N. pine plant has been successfully cultivated. Sterile vaccine

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