Arbutin and coumaric acid zero-order release quaternary compound and preparation method and application thereof
A zero-order release, complex technology, applied in applications, food science, pharmaceutical formulations, etc., can solve the problems of large fluctuations in the release rate, inability to maintain the concentration of arbutin and coumaric acid, and achieve simple operation and enhanced whitening. Effect
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Embodiment 1
[0049] A preparation method of arbutin and coumaric acid zero-order release quaternary complex, the specific steps are as follows:
[0050] (1) Dissolve 1g whey protein in 100mL ultrapure water to form a 1% (w / v) whey protein (WPC) aqueous solution; dissolve 3g high methoxyl pectin in 100mL ultrapure water to form With 3% (w / v) high methoxyl pectin (HMP) aqueous solution.
[0051] (2) Mix whey protein aqueous solution and high methoxy pectin aqueous solution at a ratio of 1:1, stir magnetically at 1000 rpm for 10 min after mixing, and adjust the pH value to 3.5 to obtain WPC-HMP complex.
[0052] (3) Store the WPC-HMP complex in a refrigerator at 4°C for 12 hours to achieve complete hydration.
[0053] (4) 20 μM arbutin and 20 μM coumaric acid were simultaneously added to 50 mL of WPC-HMP complex. The mixture was stirred at 1000 rpm for 60 min, and stood in the dark for 1 h to obtain the arbutin / coumaric acid-WPC-HMP quaternary complex.
Embodiment 2
[0055] The difference from Example 1 is that the concentration of the whey protein aqueous solution is 1% (w / v), and the concentration of the high methoxyl pectin aqueous solution is 2.5% (w / v).
Embodiment 3
[0057] The difference from Example 1 is that the concentration of the whey protein aqueous solution is 1% (w / v), and the concentration of the high methoxyl pectin aqueous solution is 3.5% (w / v).
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