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MYB gene for regulating and controlling elongation growth of cotton fiber and application thereof

A gene and gene sequence technology, applied in the field of MYB family transcription factors and their encoding genes, can solve the problems of lag in the study of the regulatory mechanism, and the function has not yet been systematically reported.

Active Publication Date: 2020-12-22
HUAZHONG NORMAL UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with Arabidopsis, the research on the regulatory mechanism of MYB transcription factors in cotton is relatively lagging behind, and its function in the process of cotton fiber elongation and development has not been systematically reported.

Method used

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  • MYB gene for regulating and controlling elongation growth of cotton fiber and application thereof
  • MYB gene for regulating and controlling elongation growth of cotton fiber and application thereof
  • MYB gene for regulating and controlling elongation growth of cotton fiber and application thereof

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Experimental program
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Embodiment Construction

[0020] 1. Genomic DNA sequence and cDNA isolation and cloning of cotton GhFEM1 gene

[0021] Cotton fiber RNA was extracted 9 days after flowering by CTAB method (according to Li XB, Cai L, Cheng NH, Liu JW, 2002. Molecular characterization of the cotton GhTUB1 gene that preferentially expressed in fiber.Plant Physiol.130:666-674), Use SuperScript TM II RNase H-Reverse Transcriptase (Invitrogen Life Technologies) was reverse transcribed into cDNA; then, using cDNA as a template, gene-specific primers (GhFEM1-ORF-L 5'-GGGGAATTCATGGATGTAACAAGCACACC-3' and GhFEM1-ORF-R 5' -CTTGGATCCTCACTCGATCTTACTCATCC-3') and high-fidelity DNA polymerase PFU, at 94°C-5 minutes, 1 cycle; 94°C-1 minute, 58°C-1 minute, 72°C-1.5 minutes, 29 cycles; finally, 72 Under the PCR reaction condition of 10 minutes extension at ℃, a DNA fragment (FEM1 gene) of about 1 kb was amplified, as shown in SEQ ID No.1. This fragment was inserted into pBluescript SK-vector. Use the T3 and T7 promoters on the plasm...

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Abstract

The invention relates to an MYB gene for regulating and controlling elongation growth of cotton fiber and application thereof. The gene is derived from gossypium hirsutum, the full length of a nucleotide sequence of the gene is 1149 bp, the gene does not contain an intron, an open reading frame of the gene is 933 bp, and the gene encodes a protein containing 310 amino acids. The transcription factor has a typical MYB domain and a transcription inhibition factor binding domain (EAR-domain). The function of the gene in regulation and control of cotton fiber elongation development is verified through genetic transformation, the length of cotton fiber can be remarkably reduced by reducing the expression quantity of the gene in the cotton fiber through an RNAi technology, it is indicated that the gene is the gene capable of positively regulating and controlling cotton fiber elongation development, the gene has important value in cotton fiber quality improvement, and the gene can be used formolecular breeding research of cotton fiber quality improvement through hybridization, transgenosis and the like.

Description

Technical field: [0001] The present invention relates to a MYB family transcription factor and its coding gene expressed predominantly during the rapid elongation period of cotton fibers, named GhFEM1 ( F iber E longation-related M YB transcription factor 1), this gene can positively regulate the fiber elongation growth of upland cotton. The FEM1 gene involved in the present invention is a gene of great value in the improvement of cotton fiber quality, and can be used in molecular breeding research for cotton fiber quality improvement through hybridization, transgenic and other technical methods. Background technique: [0002] Cotton (Gossypium hirsutum) is an important raw material for the cotton textile industry and a strategic material reserve resource, and the cotton industry plays a pivotal role in my country's national economic production. At present, my country's domestically produced "high-grade cotton" is in short supply and "low-grade cotton" is in excess, which...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/10A01H6/60
CPCC07K14/415C12N15/8218C12N15/8261
Inventor 李学宝李扬
Owner HUAZHONG NORMAL UNIV
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