A kind of animal cell hollow fiber culture system and cell culture method thereof

Abstract

The invention discloses a hollow fiber culture system for animal cells and a cell culture method thereof. The system comprises a hollow fiber cylinder, a liquid storage system, a circulation system (inner circulation system and an outer circulation system) and a detection and control system; the hollow fiber cylinder passes through the inner The circulation system circulates the medium; the external circulation system is used for cell inoculation and cell digestion, and the circulation of the peristaltic pump in the external circulation system realizes the uniform distribution of cells among the hollow fibers and improves the uniformity of cell inoculation. Establish conditions similar to in vivo growth, and the culture effect is better. The use of "alternating positive pressure circulation system (method)" ensures the alternate replacement of nutrients in the internal and external systems of mesoporous fibers, and also avoids the problems of hollow fibers being blocked and dead volumes of nutrient solutions that cannot be replaced. At the same time, the system is a closed design with good biological safety, and can effectively control pH, dissolved oxygen and temperature during the cultivation process, and is suitable for animal cell cultivation for vaccine and antibody production.

Description

technical field [0001] The invention relates to the technical field of animal cell culture, in particular to an animal cell hollow fiber culture system suitable for vaccine and antibody production and a cell culture method thereof. Background technique [0002] Commonly used cell culture methods are divided into adherent culture and suspension culture. For suspension cell culture, the scale-up of bioreactor can be used to realize the scale-up culture of suspension cells. However, when there is no suitable suspension cell line, adherent cell culture is also a vaccine. The main mode of production and antibody production. Commonly used industrial cell attachment culture methods include spinner bottle culture and fixed bed reactor culture. Spinner bottle culture is no longer approved for use in biomedicine due to its complicated operation, large differences between batches, and risks such as easy contamination. However, fixed-bed bioreactors used for adherent cell culture need ...

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Problems solved by technology

[0003] Aiming at the problem that it is difficult to realize enlarged culture due to low cell density and poor cell distribution uniformity during cell adherent culture, the present invention designs a hollow fiber culture system for animal cell adherent culture

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