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Detection kit and detection method for diabetic kidney diseases

A technology for detecting kits and kidney diseases, which is applied in the preparation of test samples, measuring devices, color/spectral characteristic measurement, etc. It can solve the problems of poor specificity, inability to detect early, low sensitivity of diabetes detection, etc.

Pending Publication Date: 2020-10-30
HUBEI JIDENGFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a detection kit and detection method for diabetic kidney disease, which has the advantages of high sensitivity, high specificity, and early detection, and solves the problem of low sensitivity and poor specificity of existing diabetes detection. , problems that cannot be detected early

Method used

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Embodiment Construction

[0042] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0043] A detection kit for diabetic kidney disease, comprising:

[0044] sealing film;

[0045] sealed bag;

[0046] Enzyme-coated plate;

[0047] Standard product: 2700ng / L;

[0048] standard diluent;

[0049] Enzyme-labeled reagents;

[0050] sample diluent;

[0051] Chromogen A solution;

[0052] Chromogen B solution;

[0053] stop solution;

[0054] Concentrate the wash solution.

[0055] Preferably, the enzyme label dilution contains 0.1M Tris, 0.05M citric acid...

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Abstract

The invention discloses a detection kit and a detection method for diabetic kidney diseases. The detection kit comprises a plate sealing film; a sealing the bag; an enzyme-labeled coating plate; a standard substance: 2700 ng / L; a standard substance diluent; an enzyme-labeled reagent; a sample diluent; a color developing agent solution A; a color developing agent solution B; a stop solution; and aconcentrated washing liquid. The invention relates to the technical field of diabetic nephropathy detection. The detection method is simple to operate, is less affected by metabolic disorders and hemodynamics, can detect diabetic nephropathy in the I phase, has extremely high sensitivity, and has great significance for clinical early diagnosis and discovery of diabetes.

Description

technical field [0001] The invention relates to the technical field of diabetic nephropathy detection, in particular to a detection kit and a detection method for diabetic nephropathy. Background technique [0002] Diabetes is a chronic metabolic disease caused by genetic and environmental factors. The main reason is that the human pancreas cannot produce insulin normally or the body cannot use insulin normally, resulting in a syndrome of higher than normal blood sugar. Diabetic nephropathy is one of the most important comorbidities of diabetic patients, which can lead to irreversible substantial damage to the kidneys: the lesions of the disease involve the small blood vessels and glomeruli in the kidneys, which can lead to the appearance of albuminuria. When a patient's diabetic nephropathy occurs, due to factors such as elevated blood sugar concentration, the glomerular filtration pressure increases and gradually leaks protein. If the condition is not controlled at this t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/78G01N21/31G01N1/38
CPCG01N1/38G01N21/01G01N21/3103G01N21/78
Inventor 洪峰
Owner HUBEI JIDENGFENG BIOTECH
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