Method for screening aluminum-toxicity-resistant oilseed rape in seed germination period

A germination stage and seed technology, which is applied in the field of aluminum toxicity-resistant rapeseed screening in the seed germination stage, can solve the problems of long time required, multi-cultivation conditions, and inability to comprehensively evaluate germplasm resources resistant to aluminum toxicity in the germination stage of rapeseed.

Inactive Publication Date: 2020-10-23
SOUTHWEST UNIV
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  • Abstract
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Problems solved by technology

[0006] The object of the present invention is to provide a kind of method that is used for the screening of aluminum-toxic rapeseed in the seed germination stage, to solve the above-mentioned background technology, the traditional analytical method in the past requires more cultivation conditions, and it takes a long time from processing to calculation results , and the analysis method is single, and it is impossible to comprehensively evaluate the germplasm resources of aluminum toxicity tolerance in the germination stage of rapeseed, which leads to the one-sidedness of the single method and the problem of poor reliability.

Method used

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  • Method for screening aluminum-toxicity-resistant oilseed rape in seed germination period

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Experimental program
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Effect test

Embodiment 1

[0063] 148 widely collected varieties (lines) of Brassica napus with different genetic backgrounds and origins were treated with aluminum toxicity stress during seed germination by the solution-filter paper method. Aluminum toxicity tolerance coefficient;

[0064] The 148 materials were divided into 3 categories, of which 10 varieties (lines) had an aluminum toxicity tolerance coefficient A greater than 1, and the highest A value was 2.02, which was a variety (line) with high aluminum toxicity tolerance; the aluminum toxicity tolerance was less than 0 There are 75 copies, the lowest A value is -1.54, which is a variety (line) sensitive to aluminum toxicity;

[0065] Sow 10 varieties (lines) with aluminum toxicity tolerance coefficient greater than 1 and 10 varieties (lines) with aluminum toxicity less than 0 in pots treated with acidic aluminum (1M) solution. Significant differences existed in growth.

Embodiment 2

[0067] According to this technical process, a recombinant inbred line population containing 188 lines (with yellow seeded high erucic acid rapeseed 10D130 (with erucic acid content higher than 50%) as the female parent and brown seeded low erucic acid rapeseed Zhongshuang 11 was treated. No. (less than 1% erucic acid content) is the male parent, and the F 2：7 population), screen out 13 strains with aluminum toxicity tolerance coefficient greater than 1 in this population;

[0068] The pot aluminum toxicity stress test verified that these 13 lines had strong tolerance to aluminum toxicity in the germination stage, which was consistent with the expected results.

Embodiment 3

[0070] According to this technical route, the self-fertile rape variety Yuyou 55 was treated, and the results showed that the aluminum toxicity tolerance coefficient of this variety was 1.12;

[0071] The variety was sown in pots in the greenhouse, and it could still germinate and grow normally after treatment with aluminum toxicity stress, showing strong tolerance to aluminum toxicity.

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Abstract

The invention belongs to the technical field of screening of aluminum-toxicity-resistant oilseed rape in a seed germination period, and particularly relates to a method for screening the aluminum-toxicity-resistant oilseed rape in the seed germination period. The method for screening the aluminum-toxicity-resistant oilseed rape in the seed germination period comprises the following steps of seed germination, investigation of main characters in the seed germination process, estimation of an aluminum toxicity tolerance comprehensive coefficient (A) and grading of aluminum-toxicity-resistant germplasms. Compared with a conventional analysis method, the method for screening the aluminum-toxicity-resistant oilseed rape in the seed germination period is simpler, more convenient and quicker, doesnot need too many culture conditions, and only needs to sow the tested oilseed rape under two culture conditions of aluminum toxicity stress and no aluminum toxicity stress (the rest conditions are consistent), count the relative germination potential, the relative germination rate, the relative fresh weight, the relative dry weight, the relative root length and the relative bud length of the tested oilseed rape, substitute the relative germination potential, the relative germination rate, the relative fresh weight, the relative dry weight, the relative root length and the relative bud lengthinto a regression equation, calculate the aluminum toxicity tolerance coefficient, and judge the aluminum toxicity tolerance of the tested oilseed rape variety (line) according to the aluminum toxicity tolerance coefficient; and 7-8 days are only needed for the process from treatment to result calculation, so that the required time is short.

Description

technical field [0001] The invention relates to the technical field of screening rapeseed resistant to aluminum toxicity in the seed germination stage, in particular to a method for screening rapeseed resistant to aluminum toxicity in the seed germination stage. Background technique [0002] The earliest and most obvious effect of aluminum toxicity on plants is to inhibit the elongation of roots. The concentration of micromolar aluminum ions can inhibit the elongation of the roots of most important crops in a short period of time. In acidic soil, it is dissociated. The aluminum ion damages the cell activity of the root meristem, inhibits cell mitosis and root growth, affects the absorption of water and nutrients, and ultimately limits the growth and yield of crops. It is used to judge whether the crop is under aluminum toxicity stress and whether it is a variety resistant to aluminum toxicity stress, but the identification of a single trait often does not take into account t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/00A01H1/04G06Q10/06G06Q50/02
CPCA01C1/00A01C1/025A01H1/04G06Q10/06393G06Q50/02
Inventor 崔翠周清元唐章林殷家明刘列钊曲存民林呐
Owner SOUTHWEST UNIV
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