An artificial binding protein capable of specifically recognizing allophycocyanin and its obtaining method
A technology of allophycocyanin and binding protein, applied in chemical instruments and methods, botany equipment and methods, biochemical equipment and methods, etc. Poor capacity and other issues
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[0080] In order to better explain the present invention and facilitate understanding, the present invention will be described in detail below through specific embodiments in conjunction with the accompanying drawings.
[0081]The overall concept of the present invention is to select highly stable, highly soluble protein A dimer as the skeleton protein, and use genetic engineering technology to treat 8-24 protein A dimers exposed on the molecular surface Randomly mutate amino acid positions to form a variable protein-binding region on the surface of the molecule, thereby constructing a backbone protein library. Then, clones that can specifically bind to target molecules are screened from the protein library by surface display technology.
[0082] Further, the method of the present invention is divided into the following steps:
[0083] S1: Phage preparation of phage display protein A dimer mutant library to obtain protein A dimer mutant library expressed on the surface of phag...
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