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Mature peptide of myotis brandti leukotriene A4 hydrolase inhibitor Motistin and application of mature peptide

A technology of leukotrienes and mature peptides of brucei bats, which is applied in the field of biomedicine to achieve the effect of inhibiting the production of inflammatory mediators

Active Publication Date: 2020-09-04
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently a lack of an inhibitor that inhibits LTA4H hydrolase activity but has no effect on its aminopeptidase activity

Method used

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  • Mature peptide of myotis brandti leukotriene A4 hydrolase inhibitor Motistin and application of mature peptide
  • Mature peptide of myotis brandti leukotriene A4 hydrolase inhibitor Motistin and application of mature peptide
  • Mature peptide of myotis brandti leukotriene A4 hydrolase inhibitor Motistin and application of mature peptide

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Embodiment 1

[0046] Example 1 Prokaryotic expression and purification of the mature peptide of the anti-inflammatory and anti-thrombotic polypeptide Motistin of Myotis brucei

[0047] 1. Construction of prokaryotic expression system

[0048] The prokaryotic expression system was constructed by Beijing Qingke Xinye Biotechnology Co., Ltd. The nucleotide sequence of the mature peptide of Motistin (as shown in SEQ ID NO: 3) was synthesized and connected to the Pet-32a recombinant plasmid, and finally transformed into into BL21 strain.

[0049] 2. Prokaryotic expression of the mature peptide of the anti-inflammatory and anti-thrombotic polypeptide Motistin of Myotis brucei

[0050] 1) Cell collection

[0051] After the obtained carrier bacteria liquid was plated to obtain a single clone, the single clone was picked and sequenced to verify the correctness of the carrier sequence, and the monoclonal bacteria were frozen at a certain concentration of glycerol.

[0052] First, a small amount of...

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Abstract

The invention provides mature peptide of a myotis brandti leukotriene A4 hydrolase inhibitor Motistin and an application of the mature peptide, and belongs to the technical field of biological medicine. The amino acid sequence of the mature peptide is shown as SEQ ID NO: 2. The invention discovers for the first time that the mature peptide of Motistin can inhibit the hydrolase activity of LTA4H without affecting the aminopeptidase activity of LTA4H. The mature peptide of Motistin can target LT44H, can inhibit the recruitment of immune cells and the generation of inflammatory mediators by inhibiting the hydrolase activity of LTA4H, and also has the function of inhibiting the generation of inflammatory cytokines IL6, TNF <alpha> and IL1<beta> caused by virus and bacterial lipopolysaccharide.The mature peptide has the potential of becoming an effective drug for inhibiting inflammation.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a mature peptide of Motistin, an inhibitor of leukotriene A4 hydrolase of Myotis brucei Brycei, and an application thereof. Background technique [0002] LTA4H is the final and rate-limiting enzyme of the arachidonic acid pathway. It is known for its epoxyhydrolase function, converting LTA4 to LTB4 (Dennis, E.A. & Norris, P.C. Eicosanoid storm in infection and inflammation. Nat Rev Immunol 15, 511–523 (2015)). [0003] LTB4 is an important pro-inflammatory lipid mediator that initiates and amplifies innate and adaptive immune responses and is chemoattractive to immune cells and releases inflammatory mediators (Peters-Golden, M. & Henderson, W.R. Leukotrienes. NEngl J Med 357, 1841–1854 (2007); DiGennaro, A. & Haeggstrom, J.Z. Theleukotrienes: immune-modulating lipid mediators of disease. Adv Immunol 116, 51-92 (2012)). Engagement of its receptors BLT1 and BLT2 has particula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N9/14C12N15/12C12N15/70C12N1/21A61K38/17A61K48/00A61P37/06A61P11/06A61P35/00A61P11/00A61P9/00A61P1/04A61P1/18A61P27/02A61P19/02A61P1/00A61P29/00A61P17/02C12R1/19
CPCA61K38/00A61K48/005A61P1/00A61P1/04A61P1/18A61P9/00A61P11/00A61P11/06A61P17/02A61P19/02A61P27/02A61P29/00A61P35/00A61P37/06C07K14/4703C12N9/14C12N15/70C12Y303/02006
Inventor 赖仞方鸣谦唐小芃张治业
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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