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Culture method of adipose-derived stem cells and application of adipose-derived stem cells in arthritis

An adipose stem cell and culture method technology, applied in the field of adipose stem cell culture, can solve problems such as side effects and limited treatment effect of arthritis, and achieve the effect of good treatment effect

Pending Publication Date: 2020-09-01
深圳安吉赛尔生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, drug treatment has more or less side effects, and because the formation mechanism of some types of arthritis such as rheumatoid arthritis is not clear, the current methods have very limited therapeutic effect on arthritis

Method used

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  • Culture method of adipose-derived stem cells and application of adipose-derived stem cells in arthritis
  • Culture method of adipose-derived stem cells and application of adipose-derived stem cells in arthritis
  • Culture method of adipose-derived stem cells and application of adipose-derived stem cells in arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for culturing fat stem cells, the specific steps are as follows:

[0044] (1) Primary culture of adipose-derived stem cells

[0045] 1) Take 5ml of adipose tissue (obtained from the subcutaneous tissue of surgical patients), and then squeeze the adipose tissue into a prepared 15ml centrifuge tube at one time, and the adipose tissue in each centrifuge tube shall not exceed 5ml;

[0046] 2) Add 7ml of buffer solution PBS into the above-mentioned centrifuge tube containing adipose tissue, pipette it 10-20 times, then let it stand for about 1min, and use the straw to suck out the liquid in the lower layer of the tissue; repeat this until the sucked out liquid is Transparent until there are no blood cells;

[0047]3) Add about 5ml of digestive solution 1 with the same volume as the tissue to the centrifuge tube with adipose tissue, seal the centrifuge tube, put it in a constant temperature shaker at 37°C, 190r / min, shake and digest for 30min; There are 3 layers, t...

Embodiment 2

[0066] A 56-year-old male suffered from grade II osteoarthritis of the right knee, and the patient's autologous adipose stem cells were cultured according to the method of Example 1, and the concentration of the adipose stem cells was 5x10 7 1 / mL, 2ml per knee joint injection, 1 injection per month, 4 injections into the articular cavity, no discomfort or discomfort after injection. After 12 months, X-rays were irradiated, and the results were as follows: figure 1 As shown, it shows that the joint space widens after 12 months after adipose stem cell treatment, indicating that the narrowing of the knee joint space caused by long-term chronic inflammation has been significantly improved, and the cartilage can be repaired.

Embodiment 3

[0068] A 45-year-old male suffers from grade II osteoarthritis cartilage defect. The patient's autologous adipose stem cells were cultured according to the method in Example 1, and the concentration of the adipose stem cells was 5×10 7 1 / mL, each knee joint was injected with 2ml each time, injected once a month, and injected into the articular cavity four times, and there was no discomfort or discomfort after the injection. After 6 months, an MRI test was performed, and the results were as follows: figure 2 As shown, the cartilage was repaired 6 months after the treatment with adipose-derived stem cells, indicating that autologous adipose-derived stem cells can repair cartilage defects.

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Abstract

The invention discloses a culture method of adipose-derived stem cells and application of the adipose-derived stem cells in arthritis. The culture method of the adipose-derived stem cells comprises primary culture of the adipose-derived stem cells, subculture of the adipose-derived stem cells, cryopreservation of the adipose-derived stem cells and resuscitation of the adipose-derived stem cells. The adipose-derived stem cells can be made into a medicine for treating arthritis, and the medicine is used for cartilage repair and chondropathy treatment and has a good treatment effect on arthritis.

Description

technical field [0001] The present invention relates to the technical field of stem cells, and more specifically relates to a method for culturing adipose stem cells and its application in arthritis. Background technique [0002] Adipose-derived stem cells (ADSCs) are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue in recent years. Adipocytes, chondrocytes, muscle cells, osteoblasts, nerve cells, glial cells and islet cells differentiate, and can secrete a variety of pro-angiogenic and anti-apoptotic factors. [0003] Adipose stem cells are a type of mesenchymal stem cells that exist in adipose tissue, accounting for about 10-20% of the total cells in subcutaneous white adipose tissue. Compared with stem cells from other sources, ADSCs have great advantages in isolation and culture: (1) Convenient sampling: adipose tissue is rich in reserves, and the sampling is less painful; currently, one liposuction can obtain 200mL of f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02A61K35/28A61P19/02A61P19/08A61P29/00
CPCC12N5/0667A01N1/0221A61K35/28A61P19/02A61P19/08A61P29/00C12N2509/00
Inventor 孔丹青
Owner 深圳安吉赛尔生物科技集团有限公司
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