Method for induction of paeonia ludlowii embryo calluses

A technology of callus induction and large flower yellow, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve problems such as low germination rate of seed embryos

Active Publication Date: 2020-08-14
TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing large-flowered yellow tree peony mainly relies on natural rhizome branching and seed propagation, while there are few studies on in vitro rapid propagation of explants, resulting in a low germination rate of seed embryos, and provides a large The Method of Callus Induction from Seed Embryo of Paeonia japonicus

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  • Method for induction of paeonia ludlowii embryo calluses
  • Method for induction of paeonia ludlowii embryo calluses
  • Method for induction of paeonia ludlowii embryo calluses

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specific Embodiment approach 1

[0020] Specific embodiment one: the method for callus induction of the seed embryo of the peony grandiflora of the present embodiment is realized according to the following steps:

[0021] 1. Collect the seeds of the large-flowered yellow peony;

[0022] 2. Preparation of mother liquor

[0023] a. Prepare a large amount of element mother liquid, which contains: KNO 3 1800~2000mg / L, NH 4 ·NO 3 1600~1700mg / L, MgSO 4 ·7H 2 O 350~400mg / L, KH 2 PO 4 160~180mg / L and CaCl 2 2H 2 O 420~460mg / L;

[0024] b. Prepare trace element mother liquor, which contains: MnSO 4 4H 2 O 20~24mg / L, ZnSO 4 ·7H 2 O 8~9mg / L, H 3 BO 3 6~6.5mg / L, KI 0.8~0.85mg / L, Na 2 MoO 4 2H 2 O 0.2~0.3mg / L, CuSO 4 ·5H 2 O 0.02~0.03mg / L and CoCl 2 ·6H 2 O 0.02~0.03mg / L;

[0025] c. Prepare iron salt mother liquor, which contains: Na 2 -EDTA 36~38mg / L and FeSO 4 ·7H 2 O 26~28mg / L;

[0026] d. Prepare organic element mother liquor, which contains: inositol 90~110mg / L, glycine 1.5~2.5mg / L, thi...

specific Embodiment approach 2

[0034] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the large amount of element mother liquor contains: KNO 3 1900mg / L, NH 4 ·NO 3 1650mg / L, MgSO 4 ·7H 2 O 370mg / L, KH 2 PO 4 170mg / L and CaCl 2 2H 2 O440mg / L.

specific Embodiment approach 3

[0035] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the trace element mother liquor contains: MnSO 4 4H 2 O 22.3mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, H 3 BO 3 6.2mg / L, KI 0.83mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O 0.025mg / L and CoCl 2 ·6H 2 O 0.025mg / L.

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Abstract

The invention provides a method for induction of paeonia ludlowii embryo calluses, belongs to the field of biotechnological breeding and aims to solve the problem that existing studies on explant in-vitro rapid propagation of paeonia ludlowii are insufficient and thus an embryo germination rate is low. The method for induction of the embryo calluses comprises: (1) collecting seeds of paeonia ludlowii; (2) preparing a major element mother liquor, a trace element mother liquor, a ferric salt mother liquor and an organic element liquor; (3) mixing and stirring the major element mother liquor, thetrace element mother liquor, the ferric salt mother liquor, the organic element liquor, sucrose and agar powder, adding plant growth regulators and preparing a culture medium; (4) removing testa andendosperm of the softened seeds and taking out mature embryos; and (5) inoculating the mature embryos on the culture medium for constant-temperature illumination cultivation. The method studies influences of the different types of plant growth regulators on in-vitro embryo growth of paeonia ludlowii, and optimizes matching ratios and concentrations of the plant growth regulators so as to increasea germination rate and a callus induction rate of the in-vitro embryos of paeonia ludlowii.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for inducing callus from embryos of peony grandiflorum. Background technique [0002] Paeonia ludlowii is endemic to Tibet. It is a perennial deciduous shrub belonging to Paeoniaceae of the Ranunculaceae family. It grows in a harsh environment, likes a mild climate, and is relatively cold-tolerant and not drought-tolerant. In the wild state, it is only distributed in the Milin and Linzhi areas of Tibet for about 70km, 2900-3200m above sea level, the valley of the Yarlung Zangbo River and the narrow strip of hillside forest edge. Its flowers are huge, beautiful in shape and bright in color. It is a rare yellow type among peonies and has a very high ornamental value. Its root bark is commonly used as a Tibetan medicinal material in the local area to treat gynecological, skin, cardiovascular and cerebrovascular diseases. Later, some researchers found that ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005Y02A40/10
Inventor 尹秀兰小中禄亚洲张二豪权红蔡皓
Owner TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
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